論文 - 詳細
| RRC ID | 1531 |
|---|---|
| 著者 | Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E. |
| タイトル | Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis. |
| ジャーナル | J Biochem |
| Abstract |
Pro-parasporin-1 is a parasporal inclusion protein of the non-insecticidal Bacillus thuringiensis strain A1190. Cytotoxic fragments, named parasporin-1, were generated from pro-parasporin-1 by trypsin digestion. Parasporin-1 was purified by a combination of chromatography procedures based on the cytotoxic activity to HeLa cells. Two different fragments of 15-kDa and 56-kDa were detected in the purified parasporin-1 fraction. These fragments were tightly associated with each other and could not be separated by chromatography under conditions that preserve cytotoxic activity, indicating that the active form of parasporin-1 is a heterodimer of the 15- and 56-kDa fragments. Amino acid sequencing and MALDI-TOF mass spectrometric analysis revealed that parasporin-1 is generated from pro-parasporin-1 by trypsin digestion at Arg 93 and Arg 231. Of 12 human cell lines tested, parasporin-1 showed strong cytotoxicity to four cell lines derived from cancer tissues, but low to no cytotoxicity to the other cell lines. The time-courses of cytotoxicity indicated that the mode of action of parasporin-1 to sensitive cells differs from that shown for previously isolated cytotoxic proteins from Bacillus thuringiensis, Cyt proteins, and other bacterial pore-forming toxins. Thus, parasporin-1 is a novel cytotoxic protein to human cancer cells produced by B. thuringiensis, and may be useful as a tool to recognize and destroy specific cancer cells. |
| 巻・号 | 137(1) |
| ページ | 17-25 |
| 公開日 | 2005-1-1 |
| DOI | 10.1093/jb/mvi003 |
| PII | 137/1/17 |
| PMID | 15713879 |
| MeSH | Animals Bacillus thuringiensis / chemistry* Bacterial Proteins / chemistry Bacterial Proteins / isolation & purification Bacterial Proteins / toxicity* Bacterial Toxins / chemistry Bacterial Toxins / isolation & purification Bacterial Toxins / toxicity* Cell Line Chlorocebus aethiops Endotoxins / chemistry Endotoxins / isolation & purification Endotoxins / toxicity* Humans Mice |
| IF | 2.476 |
| 引用数 | 48 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) Hep G2(RCB1648) Jurkat(RCB0806) Sawano(RCB1152) HL60(RCB0041) NIH3T3-3 MOLT-4(RCB0206) A549(RCB0098) CACO-2(RCB0988) MRC-5(RCB0218) COS-7(RCB0539) Vero(RCB0001) |