RRC ID 1532
著者 Yamanaka M, Eda S, Beppu M.
タイトル Carbohydrate chains and phosphatidylserine successively work as signals for apoptotic cell removal.
ジャーナル Biochem Biophys Res Commun
Abstract At an early stage of apoptosis, Jurkat cells transiently become susceptible to binding and phagocytosis by macrophages through the polylactosamine-type carbohydrate chains of CD43 [J. Biol. Chem. 279 (2004) 5967]. Susceptibility of apoptotic Jurkat cells to macrophage recognition was studied over an extended time range of 0-24 h including a later stage. Jurkat cells incubated with appropriate concentrations of apoptosis-inducing agents etoposide or anti-Fas antibody became susceptible to macrophage-binding at 2 h, and the susceptibility fell to the control level at 4 or 6 h. However, it increased again at later hours (6-24 h). Flow cytometric analyses of CD43 and phosphatidylserine (PS) on the apoptotic cells indicated that CD43 began to degrade at around 4 h, and PS is externalized significantly at 4 or 6 h. The macrophage-binding at 2 h was prevented by glycosidase treatment of Jurkat cells, but not by annexin V. Conversely, the later binding at 12 or 18 h was not prevented by glycosidase treatment, but was done so by annexin V. These results suggest that Jurkat cells become susceptible to phagocytic removal at an early stage of apoptosis by the carbohydrate-mediated mechanism, and at a later stage by the PS-mediated mechanism.
巻・号 328(1)
ページ 273-80
公開日 2005-3-4
DOI 10.1016/j.bbrc.2004.12.171
PII S0006-291X(04)02946-8
PMID 15670780
MeSH Antibodies, Monoclonal / pharmacology Antibodies, Monoclonal, Murine-Derived Antigens, CD / metabolism* Apoptosis / drug effects Apoptosis / physiology* Carbohydrate Metabolism* Cells, Cultured Etoposide / pharmacology Humans Jurkat Cells Leukosialin Macrophages / drug effects Macrophages / physiology* Phagocytosis / drug effects Phagocytosis / physiology* Phosphatidylserines / metabolism* Sialoglycoproteins / metabolism* Signal Transduction / drug effects Signal Transduction / physiology*
IF 2.985
引用数 30
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 Jurkat(RCB0806)