RRC ID 15320
Author Kobayashi M, Kouzu N, Inami A, Toyooka K, Konishi Y, Matsuoka K, Matoh T.
Title Characterization of Arabidopsis CTP:3-deoxy-D-manno-2-octulosonate cytidylyltransferase (CMP-KDO synthetase), the enzyme that activates KDO during rhamnogalacturonan II biosynthesis.
Journal Plant Cell Physiol.
Abstract In plant cells, boron (B) occurs predominantly as a borate ester associated with rhamnogalacturonan II (RG-II), but the function of this B-RG-II complex has yet to be investigated. 3-Deoxy-D-manno-2-octulosonic acid (KDO) is a specific component monosaccharide of RG-II. Mutant plants defective in KDO biosynthesis are expected to have altered RG-II structure, and would be useful for studying the physiological function of the B-RG-II complex. Here, we characterized Arabidopsis CTP:KDO cytidylyltransferase (CMP-KDO synthetase; CKS), the enzyme activating KDO as a nucleotide sugar prior to its incorporation into RG-II. Our analyses localized the Arabidopsis CKS protein to mitochondria. The Arabidopsis CKS gene occurs as a single-copy gene in the genome, and we could not obtain cks null mutants from T-DNA insertion lines. Analysis using +/cks heterozygotes in the quartet1 background demonstrated that the cks mutation rendered pollen infertile through the inhibition of pollen tube elongation. These results suggest that KDO is an indispensable component of RG-II, and that the complete B-RG-II complex is essential for the cell wall integrity of rapidly growing tissues.
Volume 52(10)
Pages 1832-43
Published 2011-10
DOI 10.1093/pcp/pcr120
PII pcr120
PMID 21893514
MeSH Amino Acid Sequence Arabidopsis / cytology Arabidopsis / enzymology* Arabidopsis / ultrastructure Chromosome Segregation / genetics DNA, Plant / genetics Genotype Germ Cells, Plant / metabolism Molecular Sequence Data Mutagenesis, Insertional / genetics Mutation / genetics Nucleotidyltransferases / chemistry Nucleotidyltransferases / metabolism* Nucleotidyltransferases / ultrastructure Pectins / biosynthesis* Pollen Tube / cytology Pollen Tube / growth & development Pollen Tube / metabolism Protein Transport Recombinant Proteins / metabolism Sequence Alignment Subcellular Fractions / enzymology Sugar Acids / metabolism*
IF 4.059
Times Cited 14
WOS Category PLANT SCIENCES CELL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes rpc00008