RRC ID 15377
Author Slováková J, Carmena A.
Title Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
Journal Development
Abstract Vertebrates and insects alike use glial cells as intermediate targets to guide growing axons. Similar to vertebrate oligodendrocytes, Drosophila midline glia ensheath and separate axonal commissures. Neuron-glia interactions are crucial during these events, although the proteins involved remain largely unknown. Here, we show that Canoe (Cno), the Drosophila ortholog of AF-6, and the DE-cadherin Shotgun (Shg) are highly restricted to the interface between midline glia and commissural axons. cno mutant analysis, genetic interactions and co-immunoprecipitation assays unveil Cno function as a novel regulator of neuron-glia interactions, forming a complex with Shg, Wrapper and Neurexin IV, the homolog of vertebrate Caspr/paranodin. Our results also support additional functions of Cno, independent of adherens junctions, as a regulator of adhesion and signaling events in non-epithelial tissues.
Volume 138(8)
Pages 1563-71
Published 2011-4-1
DOI 10.1242/dev.056192
PII dev.056192
PMID 21389054
MeSH Animals Cadherins / metabolism* Cell Adhesion Molecules, Neuronal / metabolism* Central Nervous System / cytology Central Nervous System / metabolism* Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Fluorescent Antibody Technique Immunohistochemistry Immunoprecipitation Nerve Tissue Proteins / metabolism* Neuroglia / metabolism* Neurons / metabolism* Protein Binding
IF 5.611
Times Cited 11
Drosophila Df(3R)ED5147 (DGRC#150143)