論文 - 詳細
| RRC ID | 15445 |
|---|---|
| 著者 | Povelainen M, Miasnikov AN. |
| タイトル | Production of xylitol by metabolically engineered strains of Bacillus subtilis. |
| ジャーナル | J Biotechnol |
| Abstract |
Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes were compared and it was found that the XPDH enzymes of Lactobacillus rhamnosus and Clostridium difficile had the highest selectivity towards D-xylulose 5-phosphate. Expression of these two XPDH enzymes in D-ribulose and D-xylulose producing B. subtilis strain resulted in strains of B. subtilis capable of converting D-glucose into xylitol at around 23% yield. |
| 巻・号 | 128(1) |
| ページ | 24-31 |
| 公開日 | 2007-1-30 |
| DOI | 10.1016/j.jbiotec.2006.09.008 |
| PII | S0168-1656(06)00781-4 |
| PMID | 17079043 |
| MeSH | Bacillus subtilis / enzymology Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Clostridioides difficile / enzymology Clostridioides difficile / genetics Fermentation Genetic Engineering Glucose / metabolism Lactobacillus rhamnosus / enzymology Lactobacillus rhamnosus / genetics Recombinant Proteins / metabolism* Ribulosephosphates / metabolism Sugar Alcohol Dehydrogenases / genetics* Xylitol / metabolism* Xylose / metabolism |
| IF | 3.503 |
| 引用数 | 26 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 9153 |