RRC ID 15569
Author Ulvila J, Vanha-aho LM, Kleino A, Vähä-Mäkilä M, Vuoksio M, Eskelinen S, Hultmark D, Kocks C, Hallman M, Parikka M, Rämet M.
Title Cofilin regulator 14-3-3zeta is an evolutionarily conserved protein required for phagocytosis and microbial resistance.
Journal J Leukoc Biol
Abstract Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.
Volume 89(5)
Pages 649-59
Published 2011-5-1
DOI 10.1189/jlb.0410195
PII jlb.0410195
PMID 21208897
MeSH 14-3-3 Proteins / antagonists & inhibitors 14-3-3 Proteins / genetics* Actin Depolymerizing Factors / genetics* Animals Biological Evolution* Cells, Cultured Drosophila melanogaster / genetics* Drosophila melanogaster / immunology Drosophila melanogaster / microbiology* Escherichia coli / pathogenicity* Escherichia coli Infections / genetics Escherichia coli Infections / immunology Escherichia coli Infections / microbiology Female Gene Silencing Genes, Insect Hemocytes / metabolism Male Mice Phagocytosis / physiology* RNA, Small Interfering / genetics Zebrafish / genetics Zebrafish / immunology Zebrafish / microbiology
IF 4.012
Times Cited 32
Drosophila 17870R-1 17870R-2