論文 - 詳細
RRC ID | 15625 |
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著者 | Fujii T, Takaya N. |
タイトル | Denitrification by the fungus Fusarium oxysporum involves NADH-nitrate reductase. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Fusarium oxysporum JCM11502 expresses a denitrifying (nitrate (NO(3)(-))-respiring) mechanism and can thrive under oxygen (O(2)) limitation. The fungus reduces NO(3)(-) to nitrite at the initial step of denitrification. In this study, we cloned the gene coding NADH-NO(3)(-) reductase (NADH-Nar) (niaD) from F. oxysporum JCM11502. The niaD gene complemented the defective NO(3)(-) assimilation by mutant strain M10, indicating that the fungus reduced NO(3)(-) through NADH-Nar activity and assimilated it like other fungi. We found that the transcription of niaD and the production of NADH-Nar activity were enhanced under O(2)-limited denitrifying conditions relative to aerobic conditions. Strain M10 produced less NADH-Nar activity and less denitrified product than the wild-type strain. Introducing niaD into the mutant also restored these defects, indicating that niaD is involved in denitrification. These results indicate that the fungus denitrified NO(3)(-) through NADH-Nar activity in addition to the ubiquinol-Nar mechanism. |
巻・号 | 72(2) |
ページ | 412-20 |
公開日 | 2008-2-1 |
DOI | 10.1271/bbb.70538 |
PII | JST.JSTAGE/bbb/70538 |
PMID | 18256499 |
MeSH | Base Sequence Cloning, Molecular DNA Primers DNA, Complementary Fusarium / metabolism* Genes, Fungal Genetic Complementation Test Mutation Nitrate Reductase (NADH) / genetics Nitrate Reductase (NADH) / metabolism* Nitrates / metabolism* Plasmids Reverse Transcriptase Polymerase Chain Reaction |
IF | 1.516 |
引用数 | 23 |
WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
一般微生物 | JCM 11502 |