論文 - 詳細
| RRC ID | 15726 |
|---|---|
| 著者 | Liu B, Wu L, Liu T, Hong Y, Shen Y, Ni J. |
| タイトル | A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties. |
| ジャーナル | Biotechnol Lett |
| Abstract |
A glycerate kinase gene (ST2037) from the hyperthermophilic crenarchaeon Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The purified homodimeric protein (45 kDa) specifically catalyzed the formation of 2-phosphoglycerate with D-glycerate as substrate. The thermostable enzyme displayed maximum activity (over 20 min) at 90 degrees C and pH 4.5. The maximal activity was in the presence of Co(2+). The MOFRL family glycerate kinase used AMP as phosphate donor with maximal activity towards GTP. These characteristics of the enzyme suggested its potential in the catalytic production of 2-phosphoglycerate. |
| 巻・号 | 31(12) |
| ページ | 1937-41 |
| 公開日 | 2009-12-1 |
| DOI | 10.1007/s10529-009-0089-z |
| PMID | 19690808 |
| MeSH | Adenosine Monophosphate / metabolism Cloning, Molecular Cobalt / pharmacology Coenzymes / pharmacology Dimerization Enzyme Stability Escherichia coli / genetics Gene Expression Glyceric Acids / metabolism Guanosine Triphosphate / metabolism Hydrogen-Ion Concentration Molecular Weight Phosphotransferases (Alcohol Group Acceptor) / chemistry Phosphotransferases (Alcohol Group Acceptor) / genetics* Phosphotransferases (Alcohol Group Acceptor) / isolation & purification Phosphotransferases (Alcohol Group Acceptor) / metabolism* Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Sulfolobus / enzymology* Sulfolobus / genetics* Temperature |
| IF | 1.977 |
| 引用数 | 4 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 10545 |