| RRC ID |
15739
|
| 著者 |
Yano T, Yamagami R, Misumi K, Kubota C, Moe KK, Hayashi T, Yoshitani K, Ohtake O, Misawa N.
|
| タイトル |
Genetic heterogeneity among strains of Treponema phagedenis-like spirochetes isolated from dairy cattle with papillomatous digital dermatitis in Japan.
|
| ジャーナル |
J Clin Microbiol
|
| Abstract |
Papillomatous digital dermatitis (PDD) is an infectious foot disease of cattle that is prevalent throughout the world. Although it has been prevalent in Japan since the first case was reported in 1992, full epidemiological and bacteriological examinations have not been conducted. We collected 91 lesions of PDD from 80 dairy cattle on 12 farms in eight regions of Japan to isolate the spirochetes that are frequently detected in lesions. We isolated 40 strains of spirochetes from 24 cattle (30.0%) by a simple two-step culture technique, in which the biopsy samples were incubated at 4 degrees C for 48 to 72 h in an enrichment broth supplemented with antibiotics, which improved the rate of isolation, and then inoculated on selective agar plates. All spirochetes examined were catalase positive and oxidase negative and showed weak beta-hemolytic activity. Enzyme activities were identical to those of Treponema phagedenis ATCC 27087. Sequencing of the 16S rRNA gene showed that all strains isolated had >99% identity to those of the T. phagedenis type strain and of T. phagedenis-like strains isolated from PDD lesions in the United States and Europe. Pulsed-field gel electrophoresis and PCR-based random amplified polymorphism DNA methods revealed considerable diversity among strains isolated not only from different cattle but also from the same individuals. These findings may provide further evidence for the role of these treponemes in the pathogenesis of persistent PDD.
|
| 巻・号 |
47(3)
|
| ページ |
727-33
|
| 公開日 |
2009-3-1
|
| DOI |
10.1128/JCM.01574-08
|
| PII |
JCM.01574-08
|
| PMID |
19144810
|
| PMC |
PMC2650925
|
| MeSH |
Animals
Bacterial Typing Techniques
Catalase / metabolism
Cattle
Cattle Diseases / microbiology*
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Dermatitis / microbiology
Dermatitis / veterinary*
Electrophoresis, Gel, Pulsed-Field
Genetic Heterogeneity*
Genotype
Hemolysis
Japan
Molecular Sequence Data
Oxidoreductases / metabolism
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Skin Diseases, Bacterial / microbiology
Skin Diseases, Bacterial / veterinary*
Treponema / classification*
Treponema / genetics
Treponema / isolation & purification*
Treponemal Infections / microbiology
Treponemal Infections / veterinary*
|
| IF |
5.897
|
| 引用数 |
19
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 8225 |
