RRC ID 15814
著者 Liu H, Gao Y, Yu LR, Jones RC, Elkins CA, Hart ME.
タイトル Inhibition of Staphylococcus aureus by lysostaphin-expressing Lactobacillus plantarum WCFS1 in a modified genital tract secretion medium.
ジャーナル Appl Environ Microbiol
Abstract Lactobacillus species are a predominant member of the vaginal microflora and are critical in maintaining an acidic vaginal environment thought to contribute to the prevention of a number of urogenital diseases. However, during menstruation the pH of the vaginal environment increases to neutrality, a pH conducive for Staphylococcus aureus proliferation and the production of toxic shock syndrome toxin 1 (TSST-1) in susceptible women. In order to generate Lactobacillus species capable of expressing lysostaphin (an endopeptidase that cleaves the cell wall of S. aureus) in a modified genital tract secretion medium (mGTS) under neutral-pH conditions, six prominent proteins from Lactobacillus plantarum WCFS1 spent medium were identified by mass spectrometry. Sequences for promoters, signal peptides, and mature lysostaphin were used to construct plasmids that were subsequently transformed into L. plantarum WCFS1. The promoter and signal sequences of Lp_3014 (putatively identified as a transglycosylase) or the promoter sequence of Lp_0789 (putatively identified as glyceraldehyde 3-phosphate dehydrogenase) with the signal sequence of Lp_3014 exhibited lysostaphin activity on buffered medium containing heat-killed S. aureus. The cassettes were integrated into the chromosome of L. plantarum WCFS1, but only the cassette containing the promoter and signal sequence from Lp_3014 had integrated into the appropriate site. Coculture assays using buffered mGTS showed that lysostaphin expressed from L. plantarum WCFS1 reduced the growth of TSST-1-producing strains of S. aureus under neutral-pH conditions. This study provides the basis for determining whether lysostaphin-producing Lactobacillus strains could potentially be used as a means to inhibit the growth of S. aureus during menstruation.
巻・号 77(24)
ページ 8500-8
公開日 2011-12-1
DOI 10.1128/AEM.06755-11
PII AEM.06755-11
PMID 21984245
PMC PMC3233112
MeSH Antibiosis* Culture Media / chemistry Gene Expression Humans Lactobacillus plantarum / enzymology* Lactobacillus plantarum / genetics Lysostaphin / metabolism* Organisms, Genetically Modified Plasmids Promoter Regions, Genetic Protein Sorting Signals / genetics Recombinant Proteins / genetics Recombinant Proteins / metabolism Staphylococcus aureus / drug effects Staphylococcus aureus / growth & development*
IF 4.016
引用数 9
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 2124 JCM 11305