RRC ID 15827
Author Sawai H, Na K, Sasaki N, Mimitsuka T, Minegishi S, Henmi M, Yamada K, Shimizu S, Yonehara T.
Title Membrane-integrated fermentation system for improving the optical purity of D-lactic acid produced during continuous fermentation.
Journal Biosci. Biotechnol. Biochem.
Abstract This report describes the production of highly optically pure D-lactic acid by the continuous fermentation of Sporolactobacillus laevolacticus and S. inulinus, using a membrane-integrated fermentation (MFR) system. The optical purity of D-lactic acid produced by the continuous fermentation system was greater than that produced by batch fermentation; the maximum value for the optical purity of D-lactic acid reached 99.8% enantiomeric excess by continuous fermentation when S. leavolacticus was used. The volumetric productivity of the optically pure D-lactic acid was about 12 g/L/h, this being approximately 11-fold higher than that obtained by batch fermentation. An enzymatic analysis indicated that both S. laevolacticus and S. inulinus could convert L-lactic acid to D-lactic acid by isomerization after the late-log phase. These results provide evidence for an effective bio-process to produce D-lactic acid of greater optical purity than has conventionally been achieved to date.
Volume 75(12)
Pages 2326-32
Published 2011
DOI 10.1271/bbb.110486
PII JST.JSTAGE/bbb/110486
PMID 22146719
MeSH Bacillales / cytology* Bacillales / enzymology Bacillales / metabolism* Bioreactors / microbiology* Cell Membrane / metabolism* Fermentation* L-Lactate Dehydrogenase / metabolism Lactic Acid / biosynthesis* Lactic Acid / chemistry Lactobacillus / metabolism Optical Phenomena* Stereoisomerism
IF 1.255
Times Cited 5
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 1012 JCM 1164 JCM 2513 JCM 2515 JCM 6014 JCM 6124