RRC ID 15896
Author Shin NR, Kim MS, Jung MJ, Roh SW, Nam YD, Park EJ, Bae JW.
Title Leucobacter celer sp. nov., isolated from Korean fermented seafood.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A novel, Gram-reaction-positive, aerobic, rod-shaped, non-motile bacterial strain, designated NAL101(T), was isolated from gajami-sikhae, a traditional Korean fermented seafood made of flatfish. Growth occurred at 4-45 °C, at pH 5-10 and in 0-12 % (w/v) NaCl. Optimum growth occurred at 30-37 °C, at pH 8 and in 0-1 % (w/v) NaCl. The cell-wall amino acids were 2,4-diaminobutyric acid, alanine, glycine, threonine and glutamic acid and the major fatty acids were anteiso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0). The predominant menaquinone was MK-11. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The 16S rRNA gene sequence of strain NAL101(T) showed 97.7 % similarity to that of Leucobacter chironomi MM2LB(T), its closest relative. The DNA G+C content was 68.8 mol% and DNA-DNA hybridization values with closely related strains were <22 %. Phylogenetic analyses based on 16S rRNA gene sequences as well as differences in its physiological and biochemical characteristics indicated that strain NAL101(T) represents a novel species of the genus Leucobacter in the family Microbacteriaceae, for which the name Leucobacter celer sp. nov. is proposed. The type strain is NAL101(T) ( = KACC 14220(T)  = JCM 16465(T)).
Volume 61(Pt 10)
Pages 2353-7
Published 2011-10
DOI 10.1099/ijs.0.026211-0
PII ijs.0.026211-0
PMID 21037031
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification* Actinomycetales / physiology Aerobiosis Amino Acids / analysis Bacterial Typing Techniques Base Composition Cell Wall / chemistry Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Hydrogen-Ion Concentration Korea Molecular Sequence Data Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Seafood / microbiology* Sequence Analysis, DNA Sodium Chloride / metabolism Temperature Vitamin K 2 / analysis
IF 1.932
Times Cited 12
General Microbes JCM 16465