RRC ID 15902
著者 Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Niimura Y.
タイトル Lactobacillus ozensis sp. nov., isolated from mountain flowers.
ジャーナル Int J Syst Evol Microbiol
Abstract Five strains (Mizu2-1(T), Gon2-7, Koba6-1, Koyu2-2 and Miya2-2) of lactic acid bacteria (LAB) were isolated from flowers in Oze National Park, Japan, using anaerobic cultivation. The five isolates were found to share identical 16S rRNA gene sequences. The isolates exhibited low levels of 16S rRNA gene sequence similarity to known LAB; the closest recognized relatives of strain Mizu2-1(T) were the type strains of Lactobacillus kunkeei (94.9 %), Lactobacillus kefiri (94.1 %) and Lactobacillus buchneri (93.9 %). Comparative analyses of rpoA and pheS gene sequences demonstrated that the novel isolates did not show significant relationships to other Lactobacillus species. The strains were Gram-stain-positive, catalase-negative and heterofermentative. Anaerobic growth was better than aerobic growth. The isolates utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose and fructose. On the basis of phenotypic characteristics and phylogenetic data, the isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus ozensis sp. nov. is proposed. The type strain is Mizu2-1(T) ( = JCM 17196(T)  = DSM 23829(T)).
巻・号 61(Pt 10)
ページ 2435-2438
公開日 2011-10-1
DOI 10.1099/ijs.0.027847-0
PMID 21075903
MeSH Aerobiosis Anaerobiosis Bacterial Proteins / genetics Bacterial Typing Techniques Catalase / metabolism Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fermentation Flowers / microbiology* Heterotrophic Processes Japan Lactobacillus / classification* Lactobacillus / genetics Lactobacillus / isolation & purification* Lactobacillus / physiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 14
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17196 JCM 17197 JCM 17198 JCM 17199 JCM 17200