| RRC ID |
15949
|
| 著者 |
Sato I, Shimatani K, Fujita K, Abe T, Shimizu M, Fujii T, Hoshino T, Takaya N.
|
| タイトル |
Glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Fungi that can reduce elemental sulfur to sulfide are widely distributed, but the mechanism and physiological significance of the reaction have been poorly characterized. Here, we purified elemental sulfur-reductase (SR) and cloned its gene from the elemental sulfur-reducing fungus Fusarium oxysporum. We found that NADPH-glutathione reductase (GR) reduces elemental sulfur via glutathione as an intermediate. A loss-of-function mutant of the SR/GR gene generated less sulfide from elemental sulfur than the wild-type strain. Its growth was hypersensitive to elemental sulfur, and it accumulated higher levels of oxidized glutathione, indicating that the GR/glutathione system confers tolerance to cytotoxic elemental sulfur by reducing it to less harmful sulfide. The SR/GR reduced polysulfide as efficiently as elemental sulfur, which implies that soluble polysulfide shuttles reducing equivalents to exocellular insoluble elemental sulfur and generates sulfide. The ubiquitous distribution of the GR/glutathione system together with our findings that GR-deficient mutants derived from Saccharomyces cerevisiae and Aspergillus nidulans reduced less sulfur and that their growth was hypersensitive to elemental sulfur indicated a wide distribution of the system among fungi. These results indicate a novel biological function of the GR/glutathione system in elemental sulfur reduction, which is distinguishable from bacterial and archaeal mechanisms of glutathione- independent sulfur reduction.
|
| 巻・号 |
286(23)
|
| ページ |
20283-91
|
| 公開日 |
2011-6-10
|
| DOI |
10.1074/jbc.M111.225979
|
| PII |
S0021-9258(19)49095-9
|
| PMID |
21474441
|
| PMC |
PMC3121490
|
| MeSH |
Aspergillus nidulans / genetics
Aspergillus nidulans / metabolism
Drug Resistance, Fungal / drug effects
Drug Resistance, Fungal / physiology*
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Fusarium / genetics
Fusarium / metabolism*
Glutathione / genetics
Glutathione / metabolism*
Glutathione Reductase / genetics
Glutathione Reductase / metabolism*
Mutation
Oxidation-Reduction / drug effects
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Sulfides / metabolism*
Sulfur / pharmacology*
|
| IF |
4.238
|
| 引用数 |
21
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 11502 |
