論文 - 詳細
| RRC ID | 15949 |
|---|---|
| 著者 | Sato I, Shimatani K, Fujita K, Abe T, Shimizu M, Fujii T, Hoshino T, Takaya N. |
| タイトル | Glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi. |
| ジャーナル | J Biol Chem |
| Abstract |
Fungi that can reduce elemental sulfur to sulfide are widely distributed, but the mechanism and physiological significance of the reaction have been poorly characterized. Here, we purified elemental sulfur-reductase (SR) and cloned its gene from the elemental sulfur-reducing fungus Fusarium oxysporum. We found that NADPH-glutathione reductase (GR) reduces elemental sulfur via glutathione as an intermediate. A loss-of-function mutant of the SR/GR gene generated less sulfide from elemental sulfur than the wild-type strain. Its growth was hypersensitive to elemental sulfur, and it accumulated higher levels of oxidized glutathione, indicating that the GR/glutathione system confers tolerance to cytotoxic elemental sulfur by reducing it to less harmful sulfide. The SR/GR reduced polysulfide as efficiently as elemental sulfur, which implies that soluble polysulfide shuttles reducing equivalents to exocellular insoluble elemental sulfur and generates sulfide. The ubiquitous distribution of the GR/glutathione system together with our findings that GR-deficient mutants derived from Saccharomyces cerevisiae and Aspergillus nidulans reduced less sulfur and that their growth was hypersensitive to elemental sulfur indicated a wide distribution of the system among fungi. These results indicate a novel biological function of the GR/glutathione system in elemental sulfur reduction, which is distinguishable from bacterial and archaeal mechanisms of glutathione- independent sulfur reduction. |
| 巻・号 | 286(23) |
| ページ | 20283-91 |
| 公開日 | 2011-6-10 |
| DOI | 10.1074/jbc.M111.225979 |
| PII | S0021-9258(19)49095-9 |
| PMID | 21474441 |
| PMC | PMC3121490 |
| MeSH | Aspergillus nidulans / genetics Aspergillus nidulans / metabolism Drug Resistance, Fungal / drug effects Drug Resistance, Fungal / physiology* Fungal Proteins / genetics Fungal Proteins / metabolism* Fusarium / genetics Fusarium / metabolism* Glutathione / genetics Glutathione / metabolism* Glutathione Reductase / genetics Glutathione Reductase / metabolism* Mutation Oxidation-Reduction / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Sulfides / metabolism* Sulfur / pharmacology* |
| IF | 4.238 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 11502 |