Reference - Detail
| RRC ID | 15998 |
|---|---|
| Author | Grégoire P, Fardeau ML, Joseph M, Guasco S, Hamaide F, Biasutti S, Michotey V, Bonin P, Ollivier B. |
| Title | Isolation and characterization of Thermanaerothrix daxensis gen. nov., sp. nov., a thermophilic anaerobic bacterium pertaining to the phylum "Chloroflexi", isolated from a deep hot aquifer in the Aquitaine Basin. |
| Journal | Syst Appl Microbiol |
| Abstract |
A new strictly anaerobic thermophilic multicellular filamentous bacterium (0.2-0.3μm×>100μm), designated GNS-1(T), was isolated from a deep hot aquifer in France. It was non-motile, and stained Gram-negative. Optimal growth was observed at 65°C, pH 7.0, and 2gL(-1) of NaCl. Strain GNS-1(T) was chemoorganotrophic fermenting ribose, glucose, galactose, arabinose, fructose, mannose, maltose, sucrose, xylose, raffinose, pyruvate, and xylan. Yeast extract was required for growth. The end products of glucose fermentation were lactate, acetate, CO(2), and H(2). The G+C content of the DNA was 57.6mol%. Its closest phylogenetic relative was Bellilinea caldifistulae with 92.5% similarity. Based on phylogenetic, genotypic and phenotypic characteristics, strain GNS-1(T) (DSM 23592(T), JCM 16980(T)) is proposed to be assigned to a novel species of a novel genus within the class Anaerolineae (subphylum I), phylum "Chloroflexi", Thermanaerothrix daxensis gen. nov., sp. nov. The GenBank accession number is HM596746. |
| Volume | 34(7) |
| Pages | 494-7 |
| Published | 2011-11-1 |
| DOI | 10.1016/j.syapm.2011.02.004 |
| PII | S0723-2020(11)00101-9 |
| PMID | 21621938 |
| MeSH | Acetates / metabolism Bacterial Typing Techniques Base Composition Base Sequence Carbohydrate Metabolism Cell Wall / metabolism Chloroflexi / classification* Chloroflexi / genetics Chloroflexi / growth & development Chloroflexi / isolation & purification* Chloroflexi / metabolism Fermentation France Genes, Bacterial Genes, rRNA Glucose / metabolism Gram-Negative Anaerobic Bacteria / classification* Gram-Negative Anaerobic Bacteria / genetics Gram-Negative Anaerobic Bacteria / growth & development Gram-Negative Anaerobic Bacteria / isolation & purification* Gram-Negative Anaerobic Bacteria / metabolism Hot Springs / microbiology* Hot Temperature Hydrogen / metabolism Hydrogen-Ion Concentration Lactic Acid / metabolism Microscopy, Phase-Contrast Molecular Sequence Data Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Water Microbiology |
| IF | 3.224 |
| Times Cited | 37 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Resource | |
| General Microbes | JCM 16980 |