RRC ID 15999
著者 Everest GJ, Cook AE, le Roes-Hill M, Meyers PR.
タイトル Nocardia rhamnosiphila sp. nov., isolated from soil.
ジャーナル Syst Appl Microbiol
Abstract In this study two actinomycete strains were isolated in Cape Town (South Africa), one from a compost heap (strain 202GMO(T)) and the other from within the fynbos-rich area surrounded by the horseracing track at Kenilworth Racecourse (strain C2). Based on 16S rRNA gene sequence BLAST analysis, the strains were identified as members of the genus Nocardia. Phylogenetic analysis showed that the strains clustered together and are most closely related to Nocardia flavorosea NRRL B-16176(T), Nocardia testacea JCM 12235(T), Nocardia sienata IFM 10088(T) and Nocardia carnea DSM 43397(T). This association was also supported by gyrB based phylogenetic analysis. The results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of both strains 202GMO(T) and C2 from related species. However, their high DNA relatedness showed that they belong to the same species. Strain 202GMO(T) was selected as the type strain to represent this novel species, for which the name Nocardia rhamnosiphila is proposed (=DSM 45147(T)=NRRL B-24637(T)).
巻・号 34(7)
ページ 508-12
公開日 2011-11-1
DOI 10.1016/j.syapm.2011.03.006
PII S0723-2020(11)00108-1
PMID 21596508
MeSH Bacterial Typing Techniques Base Sequence DNA Gyrase / genetics DNA, Bacterial / genetics Fatty Acids / chemistry Genes, Bacterial Genes, rRNA Genotype Microbial Sensitivity Tests Molecular Sequence Data Nocardia / chemistry Nocardia / classification* Nocardia / genetics Nocardia / isolation & purification* Nocardia / physiology Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Soil Microbiology* Species Specificity
IF 3.224
引用数 7
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 12235 JCM 12236