論文 - 詳細
| RRC ID | 16026 |
|---|---|
| 著者 | Nagamatsu G, Kosaka T, Saito S, Takubo K, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T. |
| タイトル | Tracing the conversion process from primordial germ cells to pluripotent stem cells in mice. |
| ジャーナル | Biol Reprod |
| Abstract |
To understand mechanisms underlying acquisition of pluripotency, it is critical to identify cells that can be converted to pluripotent stem cells. For this purpose, we focused on unipotent primordial germ cells (PGCs), which can be reprogrammed into pluripotent embryonic germ (EG) cells under defined conditions. Treatment of PGCs with combinations of signaling inhibitors, including inhibitors of MAP2K (MEK), GSK3B (GSK-3beta), and TGFB (TGFbeta) type 1 receptors, induced cells to enter a pluripotent state at a high frequency (12.1%) by Day 10 of culture. When we employed fluorescence-activated cell sorting to monitor conversion of candidate cells to a pluripotent state, we observed a cell cycle shift to S phase, indicating enrichment of pluripotent cells, during the early phase of EG formation. Transcriptome analysis revealed that PGCs retained expression of some pluripotent stem cell-associated genes, such as Pou5f1 and Sox2, during EG cell formation. On the other hand, PGCs lost their germ lineage characteristics and acquired expression of pluripotent stem cell markers, such as Klf4 and Eras. The overall gene expression profiles revealed by this system provide novel insight into how pluripotency is acquired in germ-committed cells. |
| 巻・号 | 86(6) |
| ページ | 182 |
| 公開日 | 2012-6-1 |
| DOI | 10.1095/biolreprod.111.096792 |
| PII | biolreprod.111.096792 |
| PMID | 22423052 |
| MeSH | Animals Benzamides Cell Culture Techniques* Diphenylamine / analogs & derivatives Flow Cytometry Gene Expression Profiling Germ Cells* Glycogen Synthase Kinase 3 / antagonists & inhibitors Kruppel-Like Factor 4 Mice Mice, Inbred BALB C Mice, Inbred ICR Mice, Nude Mice, Transgenic Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors Oligonucleotide Array Sequence Analysis Pluripotent Stem Cells* Pyridines Pyrimidines Receptors, Transforming Growth Factor beta / antagonists & inhibitors S Phase |
| IF | 3.322 |
| 引用数 | 12 |
| WOS 分野 | REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02290 |