| RRC ID |
16038
|
| Author |
Mon H, Kobayashi I, Ohkubo S, Tomita S, Lee J, Sezutsu H, Tamura T, Kusakabe T.
|
| Title |
Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1.
|
| Journal |
RNA Biol
|
| Abstract |
RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis.
|
| Volume |
9(1)
|
| Pages |
40-6
|
| Published |
2012-1-1
|
| DOI |
10.4161/rna.9.1.18084
|
| PII |
18084
|
| PMID |
22293577
|
| MeSH |
Animals
Bombyx / cytology
Bombyx / genetics*
Bombyx / metabolism
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Line
Cell Survival
Cloning, Molecular
Flow Cytometry
Gene Expression Regulation*
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Plasmids / genetics
Plasmids / metabolism
Promoter Regions, Genetic
RNA Interference*
RNA, Double-Stranded / genetics
RNA, Double-Stranded / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Transfection
|
| IF |
5.35
|
| Times Cited |
56
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
|
