論文 - 詳細
| RRC ID | 16038 |
|---|---|
| 著者 | Mon H, Kobayashi I, Ohkubo S, Tomita S, Lee J, Sezutsu H, Tamura T, Kusakabe T. |
| タイトル | Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1. |
| ジャーナル | RNA Biol |
| Abstract |
RNA interference (RNAi) is a conserved mechanism that catalyzes sequence-specific gene silencing and has been used for loss-of-function genetic screens in many organisms. Here, we demonstrated that the expression of Caenorhabditis elegans SID-1 (CeSID-1) could trigger effective gene silencing in the cultured silkworm cell line, BmN4 (BmN4-SID1). Soaking the BmN4-SID1 in dsRNA corresponding to endogenous target genes induced a significant decrease of the amount of mRNA or protein. A small amount of dsRNA was enough to silence the target gene in a few days. Overexpression of CeSID-1 did not affect the cell viability. Our results suggest that BmN4-SID1 can be used in many applications in silkworm cells and will become a valuable resource for gene analysis. |
| 巻・号 | 9(1) |
| ページ | 40-6 |
| 公開日 | 2012-1-1 |
| DOI | 10.4161/rna.9.1.18084 |
| PII | 18084 |
| PMID | 22293577 |
| MeSH | Animals Bombyx / cytology Bombyx / genetics* Bombyx / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Line Cell Survival Cloning, Molecular Flow Cytometry Gene Expression Regulation* Membrane Proteins / genetics Membrane Proteins / metabolism* Plasmids / genetics Plasmids / metabolism Promoter Regions, Genetic RNA Interference* RNA, Double-Stranded / genetics RNA, Double-Stranded / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Transfection |
| IF | 5.35 |
| 引用数 | 56 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
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