| RRC ID |
1713
|
| 著者 |
Hashimoto M, Ichimura T, Mizoguchi H, Tanaka K, Fujimitsu K, Keyamura K, Ote T, Yamakawa T, Yamazaki Y, Mori H, Katayama T, Kato J.
|
| タイトル |
Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome.
|
| ジャーナル |
Mol Microbiol
|
| Abstract |
The minimization of a genome is necessary to identify experimentally the minimal gene set that contains only those genes that are essential and sufficient to sustain a functioning cell. Recent developments in genetic techniques have made it possible to generate bacteria with a markedly reduced genome. We developed a simple system for formation of markerless chromosomal deletions, and constructed and characterized a series of large-scale chromosomal deletion mutants of Escherichia coli that lack between 2.4 and 29.7% of the parental chromosome. Combining deletion mutations changes cell length and width, and the mutant cells with larger deletions were even longer and wider than the parental cells. The nucleoid organization of the mutants is also changed: the nucleoids occur as multiple small nucleoids and are localized peripherally near the envelope. Inhibition of translation causes them to condense into one or two packed nucleoids, suggesting that the coupling of transcription and translation of membrane proteins peripherally localizes chromosomes. Because these phenotypes are similar to those of spherical cells, those may be a consequence of the morphological change. Based on the nucleoid localization observed with these mutants, we discuss the cellular nucleoid dynamics.
|
| 巻・号 |
55(1)
|
| ページ |
137-49
|
| 公開日 |
2005-1-1
|
| DOI |
10.1111/j.1365-2958.2004.04386.x
|
| PII |
MMI4386
|
| PMID |
15612923
|
| MeSH |
Cell Nucleus
Chromosome Deletion
Escherichia coli / cytology*
Escherichia coli / genetics*
Escherichia coli / growth & development
Escherichia coli Proteins / biosynthesis
Escherichia coli Proteins / physiology
Fluorescent Dyes / pharmacology
Genes, Bacterial
Genome, Bacterial*
Indoles / pharmacology
Microscopy
Protein Biosynthesis
Protein Synthesis Inhibitors / pharmacology
Staining and Labeling
Transcription, Genetic
|
| IF |
3.418
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 情報 |
E.coli |
