RRC ID |
17454
|
著者 |
Sakai T, Oishi K, Asahara T, Takada T, Yuki N, Matsumoto K, Nomoto K, Kushiro A.
|
タイトル |
M-RTLV agar, a novel selective medium to distinguish Lactobacillus casei and Lactobacillus paracasei from Lactobacillus rhamnosus.
|
ジャーナル |
Int J Food Microbiol
|
Abstract |
We developed a novel selective medium, modified-rhamnose-2,3,5-triphenyltetrazolium chloride-LBS-vancomycin agar (M-RTLV agar), that utilizes the fermentability of L-rhamnose to distinguish Lactobacillus casei and Lactobacillus paracasei from Lactobacillus rhamnosus. Whereas L. casei and L. paracasei formed red colonies on the M-RTLV agar, L. rhamnosus formed either pink-toned colonies or white colonies with a red spot. An intervention study was conducted to confirm the capability of M-RTLV agar to detect ingested L. casei when recovered from human feces. Subjects consumed one bottle daily of a fermented milk product (Yakult or Yakult Light, which contains L. casei strain Shirota; LcS) for 7 days. Diluents of the fecal samples were cultivated on M-RTLV agar. We were able to enumerate circular medium-sized red colonies, which were morphologically similar to L. casei/L. paracasei but clearly distinguishable from the remaining colonies owing to the color difference. These colonies were then subjected to enzyme-linked immunosorbent assay in order to identify the LcS. The viable counts of LcS were 6.6+/-0.7 log(10) CFU/g feces after intake of Yakult and 6.5+/-0.6 log(10) CFU/g feces after intake of Yakult Light (mean+/-SD).
|
巻・号 |
139(3)
|
ページ |
154-60
|
公開日 |
2010-5-15
|
DOI |
10.1016/j.ijfoodmicro.2010.03.019
|
PII |
S0168-1605(10)00159-5
|
PMID |
20385416
|
MeSH |
Adult
Agar / chemistry*
Colony Count, Microbial
Culture Media / chemistry*
Cultured Milk Products / microbiology
Feces / microbiology
Female
Fermentation
Humans
Lactobacillus casei / isolation & purification*
Lactobacillus rhamnosus / isolation & purification*
Male
Middle Aged
Rhamnose / chemistry
Young Adult
|
IF |
4.187
|
引用数 |
21
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 1112
JCM 1117
JCM 1152
JCM 1157 |