RRC ID 17495
Author Inoue J, Nishiumi S, Fujishima Y, Masuda A, Shiomi H, Yamamoto K, Nishida M, Azuma T, Yoshida M.
Title Autophagy in the intestinal epithelium regulates Citrobacter rodentium infection.
Journal Arch Biochem Biophys
Abstract Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been fully elucidated. In this study, we showed that the essential autophagy protein Atg7 is required for resistance to Citrobacter rodentium infection in the intestinal epithelium. Infected mice in which Atg7 had been conditionally deleted from the intestinal epithelium exhibited greater clinical evidence of disease and higher expression levels of pro-inflammatory cytokine mRNA in the large intestine. Moreover, C. rodentium clearance was reduced in the Atg7 conditional knockout mice. These results demonstrate that autophagy in intestinal epithelial cells plays an important role in host defense against C. rodentium infection and the regulation of C. rodentium infectious colitis.
Volume 521(1-2)
Pages 95-101
Published 2012-5-1
DOI 10.1016/j.abb.2012.03.019
PII S0003-9861(12)00110-5
PMID 22475450
MeSH Animals Antibodies, Bacterial / blood Autophagy* Autophagy-Related Protein 7 Base Sequence Citrobacter rodentium* / immunology Citrobacter rodentium* / pathogenicity Colitis / genetics Colitis / immunology Colitis / microbiology Colitis / pathology Cytokines / genetics DNA Primers / genetics Enterobacteriaceae Infections / genetics Enterobacteriaceae Infections / immunology Enterobacteriaceae Infections / microbiology* Enterobacteriaceae Infections / pathology* Immunoglobulin G / blood Intestinal Mucosa / immunology Intestinal Mucosa / microbiology* Intestinal Mucosa / pathology* Mice Mice, Knockout Microtubule-Associated Proteins / deficiency Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / physiology RNA, Messenger / genetics RNA, Messenger / metabolism
IF 3.391
Times Cited 25
Mice RBRC00806