| RRC ID |
17500
|
| 著者 |
Fukumoto N, Obama Y, Kitamura N, Niimi K, Takahashi E, Itakura C, Shibuya I.
|
| タイトル |
Hypoalgesic behaviors of P/Q-type voltage-gated Ca2+ channel mutant mouse, rolling mouse Nagoya.
|
| ジャーナル |
Neuroscience
|
| Abstract |
Rolling mouse Nagoya (tg(rol)) is a spontaneously occurring P/Q-type voltage-gated Ca2+ channel (VGCC) mutant mouse. A P/Q-type VGCC with the tg(rol) mutation has lower voltage sensitivity of activation, and mice with a homozygous genotype (tg(rol)/tg(rol)) but not with a heterozygous genotype (tg(rol)/+) show impaired motor coordination of the hind limbs. To investigate the roles of P/Q-type VGCC in pain sensing mechanisms, behavioral responses of adult tg(rol) mice to thermal, mechanical and chemical nociceptive stimuli were examined by the plantar, tail-flick, von Frey and formalin tests. The latency of the withdrawal response to thermal stimuli in the plantar or tail-flick tests was significantly longer in tg(rol)/tg(rol) mice than in tg(rol)/+ and wild-type (+/+) mice, and in tg(rol)/+ mice than in +/+ mice. The withdrawal response to mechanical stimuli in the von Frey test was lower in tg(rol)/tg(rol) mice than in +/+ mice. Although the licking time during the first 5 min after the formalin injection was similar among all of the three genotypes, that during 5-60 min was significantly shorter in tg(rol)/tg(rol) mice than in tg(rol)/+ and +/+ mice, and in tg(rol)/+ mice than in +/+ mice. Artificial inflammation induced by injection of complete Freund's adjuvant (CFA) into a hind paw significantly enhanced the withdrawal response recorded in the plantar and von Frey tests regardless of the mouse genotype. The CFA-enhanced response in the tg(rol)/tg(rol) mice was similar to the response in +/+ mice without the CFA injection. These results suggest that tg(rol) mutant mice show hypoalgesic responses caused by a lower sensitivity to nociceptive thermal, mechanical and chemical stimuli. It is concluded that the P/Q-type VGCC has a pro-nociceptive role and that the tg(rol) mutant mouse may be a useful tool to investigate the role of the P/Q-type VGCC in pain sensing mechanisms.
|
| 巻・号 |
160(1)
|
| ページ |
165-73
|
| 公開日 |
2009-4-21
|
| DOI |
10.1016/j.neuroscience.2009.02.032
|
| PII |
S0306-4522(09)00242-5
|
| PMID |
19248821
|
| MeSH |
Analysis of Variance
Animals
Calcium Channels, N-Type / genetics*
Freund's Adjuvant
Genotype
Hindlimb
Hot Temperature
Inflammation / chemically induced
Male
Mice
Mice, Mutant Strains
Mutation
Pain / chemically induced
Pain / physiopathology*
Pain Measurement
Pain Threshold
Physical Stimulation
Time Factors
|
| IF |
3.056
|
| 引用数 |
10
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 実験動物マウス |
RBRC00388 |
