RRC ID 17559
著者 Ródenas E, González-Aguilera C, Ayuso C, Askjaer P.
タイトル Dissection of the NUP107 nuclear pore subcomplex reveals a novel interaction with spindle assembly checkpoint protein MAD1 in Caenorhabditis elegans.
ジャーナル Mol Biol Cell
Abstract Nuclear pore complexes consist of several subcomplexes. The NUP107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a genetic disruption of NUP107 in a metazoan. Caenorhabditis elegans NUP107/npp-5 mutants display temperature-dependent lethality. Surprisingly, NPP-5 is dispensable for incorporation of most nucleoporins into nuclear pores and for nuclear protein import. In contrast, NPP-5 is essential for proper kinetochore localization of NUP133/NPP-15, another NUP107 complex member, whereas recruitment of NUP96/NPP-10C and ELYS/MEL-28 is NPP-5 independent. We found that kinetochore protein NUF2/HIM-10 and Aurora B/AIR-2 kinase are less abundant on mitotic chromatin upon NPP-5 depletion. npp-5 mutants are hypersensitive to anoxia, suggesting that the spindle assembly checkpoint (SAC) is compromised. Indeed, NPP-5 interacts genetically and physically with SAC protein MAD1/MDF-1, whose nuclear envelope accumulation requires NPP-5. Thus our results strengthen the emerging connection between nuclear pore proteins and chromosome segregation.
巻・号 23(5)
ページ 930-44
公開日 2012-3-1
DOI 10.1091/mbc.E11-11-0927
PII mbc.E11-11-0927
PMID 22238360
PMC PMC3290650
MeSH Active Transport, Cell Nucleus Animals Aurora Kinase B Aurora Kinases Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism Embryo, Nonmammalian / metabolism Kinetochores / metabolism Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism* Protein Serine-Threonine Kinases / metabolism Spindle Apparatus / genetics Spindle Apparatus / metabolism*
IF 3.791
引用数 25
WOS 分野 CELL BIOLOGY
リソース情報
線虫 npp-5(tm3039)