論文 - 詳細
| RRC ID | 17639 |
|---|---|
| 著者 | Yan C, Wang Y, Shen XY, Yang G, Jian J, Wang HS, Chen GQ, Wu Q. |
| タイトル | MicroRNA regulation associated chondrogenesis of mouse MSCs grown on polyhydroxyalkanoates. |
| ジャーナル | Biomaterials |
| Abstract |
Microbial polyhydroxyalkanoates (PHA) including poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) were found to induce chondrogenesis of mesenchymal stem cells (MSCs) and preserve chondrocytic phenotype as well as support chondrocytes-specific extracellular matrix (ECM) secretion. In this study, mouse MSCs cultured on the PHBHHx films for 24 h showed up-regulated expression of chondrogenic marker genes including aggrecan, col2, sox9, col10 and pthrp. To further illustrate this phenomonon, chondrogenesis-related microRNA expression profiling was examined by quantitative real-time PCR (RT-PCR) based on results of microRNA array obtained from comparison between mouse MSCs and mature mouse chondrocytes. Among 44 microRNAs related to chondrogenesis on microrray studies, considering only broadly-conserved microRNAs, seven differentially-expressed microRNAs were selected to study their target genes related to chondrogenesis. Two microRNAs out of the seven, namely, miR-29a and miR-29b, were revealed to directly target 3' UTR of col2a1 encoding type II collagen by dual-luciferase assay, and their activity was under the regulation of Sox9, the SRY-related high mobility group-box gene 9. For the first time microRNAs were shown to regulate the stem cell differentiation processes mediated by cell-material interactions. |
| 巻・号 | 32(27) |
| ページ | 6435-44 |
| 公開日 | 2011-9-1 |
| DOI | 10.1016/j.biomaterials.2011.05.031 |
| PII | S0142-9612(11)00550-3 |
| PMID | 21665270 |
| MeSH | 3' Untranslated Regions / genetics Animals Biomarkers / metabolism Cell Differentiation / drug effects Cell Differentiation / genetics Cell Line Cell Proliferation / drug effects Cells, Cultured Chondrocytes / cytology Chondrocytes / drug effects Chondrocytes / metabolism Chondrogenesis / drug effects Chondrogenesis / genetics* Collagen Type II / genetics Gene Expression Profiling Gene Expression Regulation / drug effects* HeLa Cells Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism* Mice Mice, Inbred C57BL MicroRNAs / genetics* MicroRNAs / metabolism Polyhydroxyalkanoates / pharmacology* Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction SOX9 Transcription Factor / metabolism |
| IF | 10.317 |
| 引用数 | 48 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |