RRC ID 17656
著者 Nagashima H, Kushiro M, Nakagawa H.
タイトル Nuclear factor-κB inhibitors alleviate nivalenol-induced cytotoxicity in HL60 cells.
ジャーナル Environ Toxicol Pharmacol
Abstract Tricothecene mycotoxins, such as nivalenol, are toxic to leukocytes. To elucidate the molecular mechanism of nivalenol toxicity, we investigated the involvement of nuclear factor-κB (NF-κB) in nivalenol-induced cytotoxicity in HL60 cells using the NF-κB inhibitors pyrrolidinedithiocarbamate (PDTC) and dexamethasone. Cells were treated with the chemicals for 24h before assays were performed. Nivalenol elicited interleukin (IL)-8 secretion. IL-8 secretion was lower in cells concomitantly treated with nivalenol and NF-κB inhibitors than with nivalenol alone. Nivalenol reduced monocyte chemotactic protein (MCP)-1 secretion. MCP-1 secretion was higher in cells concomitantly treated with nivalenol and NF-κB inhibitors than with nivalenol alone. NF-κB inhibitors thus alleviated the effects of nivalenol, indicating that NF-κB is important for nivalenol-caused changes in cytokine secretion. Nivalenol hindered cell proliferation, and dexamethasone reduced this effect, suggesting that NF-κB contributes to cell proliferation. Thus, it appears that NF-κB is involved in nivalenol-induced toxicity in HL60 cells.
巻・号 31(1)
ページ 258-61
公開日 2011-1-1
DOI 10.1016/j.etap.2010.09.014
PII S1382-6689(10)00171-7
PMID 21787693
MeSH Anti-Inflammatory Agents / pharmacology Antioxidants / pharmacology Cell Proliferation / drug effects Cell Survival / drug effects Chemokine CCL2 / metabolism Cytokines / metabolism Dexamethasone / pharmacology HL-60 Cells Humans Interleukin-8 / metabolism Mycotoxins / antagonists & inhibitors* Mycotoxins / toxicity NF-kappa B / antagonists & inhibitors NF-kappa B / physiology* Proline / analogs & derivatives Proline / pharmacology Thiocarbamates / pharmacology Trichothecenes / antagonists & inhibitors* Trichothecenes / toxicity*
IF 3.292
引用数 7
WOS 分野 PHARMACOLOGY & PHARMACY ENVIRONMENTAL SCIENCES TOXICOLOGY
リソース情報
ヒト・動物細胞 HL60(RCB0041)