論文 - 詳細
| RRC ID | 17661 |
|---|---|
| 著者 | Hiraki M, Kitajima Y, Koga Y, Tanaka T, Nakamura J, Hashiguchi K, Noshiro H, Miyazaki K. |
| タイトル | Aberrant gene methylation is a biomarker for the detection of cancer cells in peritoneal wash samples from advanced gastric cancer patients. |
| ジャーナル | Ann Surg Oncol |
| Abstract |
PURPOSE:To assess whether gene methylation in peritoneal fluid (PF) is clinically feasible for determining micrometastasis to the peritoneum in gastric cancer. METHODS:The gene methylation of BNIP3, CHFR, CYP1B1, MINT25, SFRP2, and RASSF2 were analyzed in 107 specimens of PF by quantitative methylation-specific polymerase chain reaction. All patients were placed into one of 3 groups: group A (n = 42), patients with depth of cancer invasion at muscularis propria (MP) or less than MP; group B (n = 45), depth of cancer invasion beyond the MP; and group C (n = 20), histologically diagnosed peritoneal metastasis or cancer cells cytologically defined in the peritoneal cavity. Patients in both groups A and B were diagnosed as having no cancer cells by peritoneal cytology and histology. RESULTS:The methylation status of the 6 genes was found to be significantly different among the 3 groups (group A, 0-5%; group B, 0-15%; group C, 15-45%; P < 0.01). Furthermore, the rate of positive methylation in any of the 6 genes was significantly different in each group (group A, 7%; group B, 20%; group C, 75%; P < 0.001). Three of 9 patients in group B with positive methylation in any of 6 genes experienced peritoneal recurrence. On the other hand, only 1 of 36 patients without gene methylation experienced peritoneal recurrence (P < 0.05). CONCLUSIONS:DNA methylation in PFs is a possible marker detecting occult neoplastic cells on the peritoneum. Methylation analysis along with a cytological examination might therefore improve the positive detection of cancer cells in PF of gastric cancer. |
| 巻・号 | 18(10) |
| ページ | 3013-9 |
| 公開日 | 2011-10-1 |
| DOI | 10.1245/s10434-011-1636-0 |
| PMID | 21409489 |
| MeSH | Adult Aged Aged, 80 and over Aryl Hydrocarbon Hydroxylases / genetics Biomarkers, Tumor / genetics* Cell Cycle Proteins / genetics Cytochrome P-450 CYP1B1 DNA Methylation* DNA, Neoplasm / genetics Feasibility Studies Female Gastric Mucosa / metabolism Gastric Mucosa / pathology Humans Male Membrane Proteins / genetics Middle Aged Miocamycin Neoplasm Micrometastasis Neoplasm Proteins / genetics Neoplasm Recurrence, Local / diagnosis Neoplasm Recurrence, Local / genetics* Neoplasm Staging Peritoneal Lavage* Peritoneal Neoplasms / genetics* Peritoneal Neoplasms / secondary Poly-ADP-Ribose Binding Proteins Polymerase Chain Reaction Prognosis Proto-Oncogene Proteins / genetics Stomach Neoplasms / genetics* Stomach Neoplasms / pathology Survival Rate Tumor Cells, Cultured Tumor Suppressor Proteins / genetics Ubiquitin-Protein Ligases |
| IF | 4.061 |
| 引用数 | 30 |
| WOS 分野 | SURGERY ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Kato III(RCB2088) MKN7(RCB0999) MKN45(RCB1001) MKN74(RCB1002) MKN1(RCB1003) MKN28(RCB1000) |