RRC ID 17671
Author Isshiki M, Umezawa K, Tamura H.
Title Coffee induces breast cancer resistance protein expression in Caco-2 cells.
Journal Biol Pharm Bull
Abstract Coffee is a beverage that is consumed world-wide on a daily basis and is known to induce a series of metabolic and pharmacological effects, especially in the digestive tract. However, little is known concerning the effects of coffee on transporters in the gastrointestinal tract. To elucidate the effect of coffee on intestinal transporters, we investigated its effect on expression of the breast cancer resistance protein (BCRP/ABCG2) in a human colorectal cancer cell line, Caco-2. Coffee induced BCRP gene expression in Caco-2 cells in a coffee-dose dependent manner. Coffee treatment of Caco-2 cells also increased the level of BCRP protein, which corresponded to induction of gene expression, and also increased cellular efflux activity, as judged by Hoechst33342 accumulation. None of the major constituents of coffee tested could induce BCRP gene expression. The constituent of coffee that mediated this induction was extractable with ethyl acetate and was produced during the roasting process. Dehydromethylepoxyquinomicin (DHMEQ), an inhibitor of nuclear factor (NF)-κB, inhibited coffee-mediated induction of BCRP gene expression, suggesting involvement of NF-κB in this induction. Our data suggest that daily consumption of coffee might induce BCRP expression in the gastrointestinal tract and may affect the bioavailability of BCRP substrates.
Volume 34(10)
Pages 1624-7
Published 2011-1-1
DOI 10.1248/bpb.34.1624
PII JST.JSTAGE/bpb/34.1624
PMID 21963506
MeSH ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters / analysis ATP-Binding Cassette Transporters / biosynthesis ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism* Benzamides / pharmacology Benzimidazoles / pharmacology Breast Neoplasms / genetics Breast Neoplasms / metabolism* Caco-2 Cells Cell Culture Techniques Coffee* Cyclohexanones / pharmacology Dose-Response Relationship, Drug Drug Evaluation, Preclinical Female Gene Expression / drug effects Humans Molecular Targeted Therapy NF-kappa B / antagonists & inhibitors NF-kappa B / genetics Neoplasm Proteins / analysis Neoplasm Proteins / biosynthesis* Neoplasm Proteins / genetics* Neoplasm Proteins / metabolism Plant Extracts / pharmacology*
IF 1.863
Times Cited 7
Human and Animal Cells CACO-2(RCB0988)