Reference - Detail
| RRC ID | 17674 |
|---|---|
| Author | Gerelchuluun A, Hong Z, Sun L, Suzuki K, Terunuma T, Yasuoka K, Sakae T, Moritake T, Tsuboi K. |
| Title | Induction of in situ DNA double-strand breaks and apoptosis by 200 MeV protons and 10 MV X-rays in human tumour cell lines. |
| Journal | Int J Radiat Biol |
| Abstract |
PURPOSE:To clarify the properties of clinical high-energy protons by comparing with clinical high-energy X-rays. MATERIALS AND METHODS:Human tumor cell lines, ONS76 and MOLT4, were irradiated with 200 MeV protons or 10 MV X-rays. In situ DNA double-strand breaks (DDSB) induction was evaluated by immunocytochemical staining of phosphorylated histone H2AX (γ-H2AX). Apoptosis was measured by flow-cytometry after staining with Annexin V. The relative biological effectiveness (RBE) was obtained by clonogenic survival assay. RESULTS:DDSB induction was significantly higher for protons than X-rays with average ratios of 1.28 (ONS76) and 1.59 (MOLT4) at 30 min after irradiation. However the differences became insignificant at 6 h. Also, apoptosis induction in MOLT4 cells was significantly higher for protons than X-rays with an average ratio of 2.13 at 12 h. However, the difference became insignificant at 20 h. RBE values of protons to X-rays at 10% survival were 1.06 ± 0.04 and 1.02 ± 0.15 for ONS76 and MOLT4, respectively. CONCLUSIONS:Cell inactivation may differ according to different timings and/or endpoints. Proton beams demonstrated higher cell inactivation than X-rays in the early phases. These data may facilitate the understanding of the biological properties of clinical proton beams. |
| Volume | 87(1) |
| Pages | 57-70 |
| Published | 2011-1-1 |
| DOI | 10.3109/09553002.2010.518201 |
| PMID | 20954835 |
| MeSH | Apoptosis / radiation effects* Cell Line, Tumor DNA Breaks, Double-Stranded* Dose-Response Relationship, Radiation Histones / metabolism Humans Medulloblastoma / metabolism Medulloblastoma / pathology Medulloblastoma / radiotherapy Particle Accelerators Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / metabolism Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / pathology Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / radiotherapy Protons* Radiotherapy, High-Energy / instrumentation Relative Biological Effectiveness Synchrotrons Tumor Stem Cell Assay |
| IF | 2.368 |
| Times Cited | 31 |
| WOS Category | NUCLEAR SCIENCE & TECHNOLOGY BIOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) |