RRC ID 18095
著者 Pritchard S, Wick HC, Slonim DK, Johnson KL, Bianchi DW.
タイトル Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung.
ジャーナル Biol Reprod
Abstract During pregnancy, cells from each fetus travel into the maternal circulation and organs, resulting in the development of microchimerism. Identification of the cell types in this microchimeric population would permit better understanding of possible mechanisms by which they affect maternal health. However, comprehensive analysis of fetal cells has been hampered by their rarity. In this study, we sought to overcome this obstacle by combining flow cytometry with multidimensional gene expression microarray analysis of fetal cells isolated from the murine maternal lung during late pregnancy. Fetal cells were collected from the lungs of pregnant female mice. cDNA was amplified and hybridized to gene expression microarrays. The resulting fetal cell core transcriptome was interrogated using multiple methods including Ingenuity Pathway Analysis, the BioGPS gene expression database, principal component analysis, the Eurexpress gene expression atlas, and primary literature. Here we report that small numbers of fetal cells can be flow sorted from the maternal lung, facilitating discovery-driven gene expression analysis. We additionally show that gene expression data can provide functional information about fetal cells. Our results suggest that fetal cells in the murine maternal lung are a mixed population, consisting of trophoblasts, mesenchymal stem cells, and cells of the immune system. Detection of trophoblasts and immune cells in the maternal lung may facilitate future mechanistic studies related to the development of immune tolerance and pregnancy-related complications, such as pre-eclampsia. Furthermore, the presence and persistence of mesenchymal stem cells in maternal organs may have implications for long-term postpartum maternal health.
巻・号 87(2)
ページ 42
公開日 2012-8-1
DOI 10.1095/biolreprod.112.101147
PII biolreprod.112.101147
PMID 22674387
PMC PMC3431427
MeSH Animals Chimerism* Female Flow Cytometry Gene Expression Profiling Lung / cytology* Male Mesenchymal Stem Cells / cytology Mice Mice, Inbred C57BL Nucleic Acid Amplification Techniques Placenta / cytology Pregnancy Pregnancy, Animal* / immunology Principal Component Analysis Transcriptome
IF 3.322
引用数 13
WOS 分野 REPRODUCTIVE BIOLOGY
リソース情報
実験動物マウス RBRC00267