論文 - 詳細
| RRC ID | 18125 |
|---|---|
| 著者 | Yang D, Yaguchi T, Nagata T, Gotoh A, Dovat S, Song C, Nishizaki T. |
| タイトル | AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis. |
| ジャーナル | Cell Physiol Biochem |
| Abstract |
BACKGROUND/AIMS:The mechanism underlying extracellular adenosine-induced caspase-independent apoptosis in HuH-7 human hepatoma cells is not fully understood. The present study investigated the role for apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death (AMID) in the pathway. METHODS:To see the implication of AMID in adenosine-induced HuH-7 cell apoptosis, real-time reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescent cytochemistry, time-laps GFP monitoring, cell cycle analysis, flow cytometry, Western blotting, cell viability assay, and TUNEL staining were carried out. RESULTS:Adenosine upregulated AMID expression in HuH-7 cells, and translocated AMID from the cytosol into the nucleus. Adenosine induced HuH-7 cell apoptosis, and the effect was further enhanced by overexpressing AMID. Adenosine-induced HuH-7 cell apoptosis, alternatively, was inhibited by knocking-down AMID. CONCLUSION:The results of the present study provide evidence for AMID as a critical factor for adenosine-induced caspase-independent HuH-7 cell apoptosis. |
| 巻・号 | 27(1) |
| ページ | 37-44 |
| 公開日 | 2011-1-1 |
| DOI | 10.1159/000325203 |
| PII | 000325203 |
| PMID | 21325820 |
| PMC | PMC3221265 |
| MeSH | Adenosine / metabolism* Apoptosis* Apoptosis Regulatory Proteins / analysis Apoptosis Regulatory Proteins / genetics Apoptosis Regulatory Proteins / metabolism* Caspases / metabolism* Cell Line, Tumor Humans Mitochondrial Proteins / analysis Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* RNA Interference RNA, Small Interfering / metabolism Up-Regulation |
| IF | 5.5 |
| 引用数 | 13 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1366) |