| RRC ID |
18130
|
| Author |
Herlambang S, Kumagai M, Nomoto T, Horie S, Fukushima S, Oba M, Miyazaki K, Morimoto Y, Nishiyama N, Kataoka K.
|
| Title |
Disulfide crosslinked polyion complex micelles encapsulating dendrimer phthalocyanine directed to improved efficiency of photodynamic therapy.
|
| Journal |
J Control Release
|
| Abstract |
Dendrimer phthalocyanine (DPc)-loaded polyion complex micelle (DPc/m) has been developed as photosensitizer (PS) formulation in photodynamic therapy (PDT). Incorporation of DPc into the micelle showed significant enhancement in the in vitro photocytotoxicity. Also, introduction of disulfide crosslinking in the micellar core further improved the in vitro PDT effect of DPc/m. Here, we aim to analyze the mechanism of the enhanced photocytotoxicity of DPc/m, particularly focusing on the photochemical reactions during photoirradiation. As a result, DPc/m has been shown to protect DPc from photobleaching induced by the reactions with serum proteins, although DPc were considerably quenched in the micellar core. Furthermore, the introduction of disulfide crosslinking into the micellar core has demonstrated to improve the efficiency of reactive oxygen species (ROS) production by DPc in the micellar core as well as more effectively prevent the photobleaching of DPc. These effects might lead to effective photochemical reactions by DPc/m, which may account for the enhanced photocytotoxicity. Our findings provide useful knowledge in designing PS formulations for effective PDT.
|
| Volume |
155(3)
|
| Pages |
449-57
|
| Published |
2011-11-7
|
| DOI |
10.1016/j.jconrel.2011.06.019
|
| PII |
S0168-3659(11)00407-X
|
| PMID |
21704092
|
| MeSH |
Cell Culture Techniques
Cell Line, Tumor
Cell Survival / drug effects
Cell Survival / radiation effects
Cross-Linking Reagents / chemistry*
Dendrimers / administration & dosage*
Dendrimers / chemistry
Dendrimers / pharmacology
Disulfides / chemistry*
Drug Compounding
Endocytosis
Humans
Indoles / administration & dosage*
Indoles / chemistry
Indoles / pharmacology
Isoindoles
Light
Micelles
Mitochondria / drug effects
Mitochondria / metabolism
Mitochondria / radiation effects
Molecular Structure
Photobleaching
Photochemotherapy / methods*
Photosensitizing Agents / administration & dosage*
Photosensitizing Agents / chemistry
Photosensitizing Agents / pharmacology
Reactive Oxygen Species / metabolism
Scattering, Radiation
Spectrophotometry, Ultraviolet
Surface Properties
|
| IF |
7.727
|
| Times Cited |
58
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| Human and Animal Cells |
A549(RCB0098) |
