RRC ID 18173
Author Wei K, Li Y, Mugishima H, Teramoto A, Abe K.
Title Fabrication of core-sheath structured fibers for model drug release and tissue engineering by emulsion electrospinning.
Journal Biotechnol J
Abstract A nanofibrous core-sheath structured scaffold incorporated with bioactive agents is supposed to promote cell migration, proliferation, and gene expressions through the controllable and sustainable release of bioactive agents from the fibers and the preservation of bioactivity. Here we present a novel and effective emulsion electrospinning method for obtaining fluorescein isothiocyanate-dextran (FITC-dextran)/poly(lactic-co-glycolic acid) (PLGA) and type I collagen/PLGA fibrous composite scaffolds. Core-sheath structured fibers with average diameters of 665 nm for FITC-dextran/PLGA and 567 nm for collagen/PLGA were successfully fabricated. In vitro-release profile shows sustained release of encapsulated FITC-dextran from FITC-dextran/PLGA fibers for as long as 7 weeks. The osteoblastic activity of the collagen/PLGA nanofibrous scaffold was investigated employing the osteoblastic-like MC3T3-E1 cell line. The results of the lactate dehydrogenase assay suggested excellent cytocompatibility. Cell proliferation and alkaline phosphatase activity were also ameliorated on this emulsion-electrospun collagen/PLGA fibrous scaffold. All the results indicated that this composite scaffold could support the early stages of osteoblast behavior as well as the immediate/late stages. The emulsion electrospinning process has potential for application in drug-release devices and as a 3-D scaffold in bone regeneration.
Volume 7(5)
Pages 677-85
Published 2012-5
DOI 10.1002/biot.201000473
PMID 22125296
MeSH Alkaline Phosphatase / chemistry Animals Biocompatible Materials / chemistry* Biocompatible Materials / pharmacology Biotechnology / methods Cattle Cell Adhesion / drug effects Cell Proliferation / drug effects Collagen Type I / chemistry Collagen Type I / pharmacology Dextrans / chemistry Dextrans / pharmacokinetics Drug Delivery Systems Emulsions / chemistry Fluorescein-5-isothiocyanate / analogs & derivatives Fluorescein-5-isothiocyanate / chemistry Fluorescein-5-isothiocyanate / pharmacokinetics Lactic Acid / chemistry Mice Nanofibers / chemistry* Osteoblasts / cytology Osteoblasts / drug effects Polyglycolic Acid / chemistry Polylactic Acid-Polyglycolic Acid Copolymer Tissue Engineering / methods* Tissue Scaffolds / chemistry*
IF 3.543
Times Cited 11
Human and Animal Cells MC3T3-E1 (RCB1126)