論文 - 詳細
| RRC ID | 18179 |
|---|---|
| 著者 | Haque A, Koide N, Iftakhar-E-Khuda I, Noman AS, Odkhuu E, Badamtseren B, Naiki Y, Komatsu T, Yoshida T, Yokochi T. |
| タイトル | Flavopiridol inhibits lipopolysaccharide-induced TNF-α production through inactivation of nuclear factor-κB and mitogen-activated protein kinases in the MyD88-dependent pathway. |
| ジャーナル | Microbiol Immunol |
| Abstract |
Flavopiridol is a cyclin-dependent kinase inhibitor and inhibits the growth of various cancer cells. The effect of flavopiridol on lipopolysaccharide (LPS)-induced proinflammatory mediator production was examined in RAW 264.7 macrophage-like cells. Flavopiridol significantly reduced the production of tumor necrosis factor-α and, to a lesser extent, nitric oxide in LPS-stimulated cells. Flavopiridol inhibited the activation of nuclear factor-κB and IκB kinase in response to LPS. Flavopiridol also inhibited the activation of a series of mitogen-activated protein kinases, such as p38, stress-activated protein kinase/c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2 in response to LPS. However, flavopiridol did not alter the expression of tumor necrosis factor receptor-associated factor 6, myeloid differentiation factor 88 (MyD88) or CD14/toll-like receptor (TLR) 4. Flavopiridol inhibited nitric oxide production induced by a MyD88-dependent TLR2 ligand, but not a MyD88-independent TLR3 ligand. Further, flavopiridol did not alter the phosphorylation of interferon regulatory factor 3 in the MyD88-independent pathway. Therefore, it was suggested that flavopiridol exclusively inhibited the activation of nuclear factor-κB and mitogen-activated protein kinases in the MyD88-dependent pathway. Flavopiridol might be useful for the prevention of LPS-induced inflammatory response. |
| 巻・号 | 55(3) |
| ページ | 160-7 |
| 公開日 | 2011-3-1 |
| DOI | 10.1111/j.1348-0421.2010.00304.x |
| PMID | 21204955 |
| MeSH | Animals Cell Line Flavonoids / pharmacology* Gene Expression Regulation, Archaeal / drug effects* Lipopolysaccharide Receptors / metabolism Lipopolysaccharides / metabolism* Macrophages / drug effects Mice Mitogen-Activated Protein Kinases / metabolism* Myeloid Differentiation Factor 88 / metabolism* NF-kappa B / metabolism* Nitric Oxide / biosynthesis Phosphorylation / drug effects Piperidines / pharmacology* Protein Kinase Inhibitors / pharmacology* Signal Transduction / drug effects TNF Receptor-Associated Factor 6 / metabolism Toll-Like Receptors / metabolism Tumor Necrosis Factor-alpha / biosynthesis* |
| IF | 1.566 |
| 引用数 | 8 |
| WOS 分野 | MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |