RRC ID 18198
Author Choi EM.
Title Deoxyactein stimulates osteoblast function and inhibits bone-resorbing mediators in MC3T3-E1 cells.
Journal J Appl Toxicol
Abstract Osteoporosis is a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue, with a consequent increase in bone fragility and susceptibility to fracture. In order to improve the treatment of osteoporosis, identification of anabolic agents with minimal side effects is highly desirable. Cimicifuga racemosa has a long and diverse history of medicinal use and deoxyactein isolated from this species is one of the major constituents. In the present study, the effect of deoxyactein on the function of osteoblastic MC3T3-E1 cells was studied. Deoxyactein caused a significant elevation of cell growth, alkaline phosphatase activity, collagen content, and mineralization in the cells (P < 0.05). Moreover, deoxyactein significantly (P < 0.05) decreased the production of reactive oxygen species (ROS) and osteoclast differentiation-inducing factors such as TNF-α, IL-6 and receptor activator of nuclear factor-κB ligand in the presence of antimycin A, which inhibits mitochondrial electron transport and has been used as an ROS generator. These results demonstrate that deoxyactein may have positive effects on skeletal structure.
Volume 33(3)
Pages 190-5
Published 2013-3-1
DOI 10.1002/jat.1733
PMID 21910134
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Antimycin A / pharmacology Bone Density Conservation Agents / pharmacology* Calcinosis Cimicifuga / chemistry* Collagen / metabolism Drug Combinations Interleukin-6 / metabolism Mesenchymal Stem Cells / drug effects* Mesenchymal Stem Cells / metabolism Mesenchymal Stem Cells / pathology Mice Mice, Inbred C57BL Osteoblasts / drug effects* Osteoblasts / metabolism Osteoblasts / pathology Oxidative Stress / drug effects Plant Extracts / toxicity RANK Ligand / metabolism Reactive Oxygen Species / metabolism Saponins / pharmacology* Triterpenes / pharmacology* Tumor Necrosis Factor-alpha / metabolism
IF 3.065
Times Cited 8
WOS Category TOXICOLOGY
Resource
Human and Animal Cells MC3T3-E1 (RCB1126)