Reference - Detail
| RRC ID | 18200 |
|---|---|
| Author | Arisaka M, Takano K, Negishi Y, Arima H, Aramaki Y. |
| Title | Involvement of lipid rafts in macrophage apoptosis induced by cationic liposomes. |
| Journal | Arch Biochem Biophys |
| Abstract |
We have demonstrated that protein kinase Cδ (PKCδ) could be involved in macrophage apoptosis induced by cationic liposomes composed of stearylamine (SA-liposomes), but the detailed mechanism of how SA-liposomes activate PKCδ has remained unclear. In this paper, we clarified whether lipid rafts are involved in the PKCδ activation induced by SA-liposomes. Co-localization of SA-liposomes and Cholera toxin B subunit (CBT), which specifically binds to ganglioside GM1 on lipid rafts, was found by microscopic observation. The incorporation of SA-liposomes into lipid rafts was clearly inhibited by the pretreatment of cells with an agent, 2,6-di-O-methyl-α-cyclodextrin (DM-α-CD) which disrupts lipid rafts. Activation of PKCδ and externalization of phosphatidylserine induced by SA-liposomes were also suppressed by DM-α-CD, which extracts sphingolipids and proteins from lipid rafts. Reactive oxygen species (ROS) generation, which could be involved in the macrophage apoptosis, was also inhibited by DM-α-CD. Furthermore, apoptosis induced by SA-liposomes was clearly inhibited when the cells were pre-treated with DM-α-CD, but not nystatin, a cholesterol-sequestering agent that disrupt lipid rafts. These findings suggest that sphingolipids in lipid rafts are involved in the activation of PKCδ which leads to apoptosis induced by cationic liposomes, SA-liposomes. |
| Volume | 508(1) |
| Pages | 72-7 |
| Published | 2011-4-1 |
| DOI | 10.1016/j.abb.2011.02.003 |
| PII | S0003-9861(11)00060-9 |
| PMID | 21315683 |
| MeSH | Amines / pharmacology Animals Apoptosis / drug effects* Cell Line Enzyme Activation / drug effects Liposomes / chemistry* Liposomes / pharmacology* Macrophages / cytology* Macrophages / drug effects* Macrophages / metabolism Membrane Microdomains / drug effects* Membrane Microdomains / metabolism* Mice Protein Kinase C-delta / metabolism Reactive Oxygen Species / metabolism |
| IF | 3.391 |
| Times Cited | 12 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |