| RRC ID |
18518
|
| Author |
Uto T, Qin GW, Morinaga O, Shoyama Y.
|
| Title |
17-Hydroxy-jolkinolide B, a diterpenoid from Euphorbia fischeriana, inhibits inflammatory mediators but activates heme oxygenase-1 expression in lipopolysaccharide-stimulated murine macrophages.
|
| Journal |
Int Immunopharmacol
|
| Abstract |
Jolkinolides are the main abietane-type diterpenoids isolated from the root of Euphorbia fischeriana Steud. In the present study, we investigated in vitro anti-inflammatory activity of four structural analogs of jolkinolide in lipopolysaccharide (LPS)-stimulated RAW264 macrophages. Among these jolkinolides, 17-hydroxy-jolkinolide B (HJB) exhibited the most potent inhibition of LPS-induced production of inflammatory mediators such as prostaglandin E(2) (PGE(2)), nitric oxide (NO), and pro-inflammatory cytokines [interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)]. HJB could decrease LPS-induced protein levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) and the mRNA expressions of COX-2, iNOS, IL-6, and TNF-α in a concentration-dependent manner. These inhibitory effects were caused by suppression of MAPK phosphorylation and NF-κB activation. Furthermore, we demonstrated that HJB strongly induced heme oxygenase-1 (HO-1) protein and mRNA expressions. These findings suggest that HJB possesses anti-inflammatory actions in macrophages and may provide a potential therapeutic approach for inflammatory disorders.
|
| Volume |
12(1)
|
| Pages |
101-9
|
| Published |
2012-1-1
|
| DOI |
10.1016/j.intimp.2011.10.020
|
| PII |
S1567-5769(11)00418-8
|
| PMID |
22080918
|
| MeSH |
Animals
Anti-Inflammatory Agents / pharmacology*
Cell Line
Cells, Cultured
Cyclooxygenase 2 / genetics
Dinoprostone / metabolism
Diterpenes / pharmacology*
Down-Regulation
Drugs, Chinese Herbal / pharmacology*
Euphorbia
Female
Heme Oxygenase-1 / genetics
Heme Oxygenase-1 / metabolism*
I-kappa B Kinase / metabolism
Interleukin-6 / genetics
Lipopolysaccharides
Macrophages / drug effects*
Macrophages / metabolism
Mice
Mice, Inbred C57BL
Mitogen-Activated Protein Kinases / metabolism
NF-kappa B / metabolism
Nitric Oxide / metabolism
Nitric Oxide Synthase Type II / genetics
Plant Roots
RNA, Messenger / metabolism
Tumor Necrosis Factor-alpha / genetics
|
| IF |
3.943
|
| Times Cited |
21
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
