| RRC ID |
18562
|
| 著者 |
Nakagami G, Minematsu T, Asada M, Nagase T, Akase T, Huang L, Morohoshi T, Ikeda T, Ohta Y, Sanada H.
|
| タイトル |
The Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl) homoserine lactone can accelerate cutaneous wound healing through myofibroblast differentiation in rats.
|
| ジャーナル |
FEMS Immunol Med Microbiol
|
| Abstract |
Quorum sensing is a cell density-dependent gene regulation system in bacteria. N-(3-oxododecanoyl) homoserine lactone (3-oxo-C12-HSL) is used in the las quorum-sensing system in Pseudomonas aeruginosa, which is an opportunistic pathogen that causes many human diseases. Although many studies have investigated the sole effects of quorum sensing on several types of mammalian cells, including lung cells, little is known about the effects of quorum sensing on the cells associated with wound healing. To better understand the mechanism of bacterial wound infection, we investigated the effects of 3-oxo-C12-HSL on cells using a rat full-thickness wound-healing model. We found that the wound contraction was significantly increased at 24 h after the administration of 3-oxo-C12-HSL to the surface of granulation tissue. Differentiation of fibroblasts to myofibroblasts was induced in the in vivo wound-healing model and was confirmed in vitro using the rat fibroblastic cell line Rat-1. Cyclooxygenase (Cox)-2 expression was also induced in Rat-1 cells by 3-oxo-C12-HSL. This finding suggested that Cox-2 upregulation may be related to the inflammatory findings in the histological examinations, in which infiltrating polymorphonuclear neutrophils were observed at the wound site. Taken together, these results imply that mammals have a potential defense system against invading pathogens by responding to the presence of 3-oxo-C12-HSL and inducing the differentiation of fibroblasts to myofibroblasts as well as inflammation for accelerating wound healing.
|
| 巻・号 |
62(2)
|
| ページ |
157-63
|
| 公開日 |
2011-7-1
|
| DOI |
10.1111/j.1574-695X.2011.00796.x
|
| PMID |
21457165
|
| MeSH |
4-Butyrolactone / analogs & derivatives*
4-Butyrolactone / metabolism
Animals
Cell Differentiation
Cells, Cultured
Cyclooxygenase 2 / biosynthesis
Disease Models, Animal
Gene Expression
Histocytochemistry
Homoserine / analogs & derivatives*
Homoserine / metabolism
Inflammation / pathology
Male
Myofibroblasts / drug effects*
Neutrophils / immunology
Pseudomonas aeruginosa / metabolism*
Rats
Rats, Sprague-Dawley
Up-Regulation
Wound Healing / drug effects*
Wounds and Injuries / pathology
|
| 引用数 |
13
|
|
WOS 分野
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Rat-1(RCB1830) |
