Reference - Detail
| RRC ID | 18564 |
|---|---|
| Author | Sawai H, Domae N. |
| Title | Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Apoptotic cell death eventually results in secondary necrotic cell death, whereas caspase-independent primary necrotic cell death has been reported and its mechanism involving RIP1 and RIP3 has been recently elucidated. Dual staining with fluorescent Annexin V and propidium iodide (PI) has been used to discriminate apoptotic and necrotic cell death, in which Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis. Here we demonstrate that primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive, and that primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1, an inhibitor of primary necrosis by inhibition of RIP1. |
| Volume | 411(3) |
| Pages | 569-73 |
| Published | 2011-8-5 |
| DOI | 10.1016/j.bbrc.2011.06.186 |
| PII | S0006-291X(11)01199-5 |
| PMID | 21763280 |
| MeSH | Annexin A5 / analysis Annexin A5 / metabolism* Apoptosis / drug effects* Caspases / metabolism Coloring Agents / chemistry* Humans Imidazoles / pharmacology* Indoles / pharmacology* Necrosis Nuclear Pore Complex Proteins / antagonists & inhibitors* Propidium / chemistry* RNA-Binding Proteins / antagonists & inhibitors* Staining and Labeling U937 Cells |
| IF | 2.985 |
| Times Cited | 94 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | U-937 DE-4(RCB0435) |