RRC ID 18574
著者 Otsu K, Kishigami R, Oikawa-Sasaki A, Fukumoto S, Yamada A, Fujiwara N, Ishizeki K, Harada H.
タイトル Differentiation of induced pluripotent stem cells into dental mesenchymal cells.
ジャーナル Stem Cells Dev
Abstract Similar to embryonic stem cells, induced pluripotent stem (iPS) cells can differentiate into various cell types upon appropriate induction, and thus, may be valuable cell sources for regenerative medicine. However, iPS cells have not been reported to differentiate into odontogenic cells for tooth regeneration. Here we demonstrated that neural crest-like cells (NCLC) derived from mouse iPS cells have the potential to differentiate into odontogenic mesenchymal cells. We developed an efficient culture protocol to induce the differentiation of mouse iPS cells into NCLC. We confirmed that the cells exhibited neural crest (NC) cell markers as evidenced by immunocytochemistry, flow cytometry, and real-time reverse transcription-polymerase chain reaction. Further, in recombination cultures of NCLC and mouse dental epithelium, NCLC exhibited a gene expression pattern involving dental mesenchymal cells. Some NCLC also expressed dentin sialoprotein. Conditioned medium of mouse dental epithelium cultures further enhanced the differentiation of NCLC into odontoblasts. These results suggest that iPS cells are useful cell sources for tooth regeneration and tooth development studies.
巻・号 21(7)
ページ 1156-64
公開日 2012-5-1
DOI 10.1089/scd.2011.0210
PMID 22085204
MeSH Animals Biomarkers / metabolism Cell Differentiation* Cell Transformation, Neoplastic Cells, Cultured Coculture Techniques Epithelial Cells / metabolism Gene Expression Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / physiology* Induced Pluripotent Stem Cells / transplantation Mice Mice, Nude Neural Crest / metabolism Odontoblasts / metabolism Odontogenesis Teratoma / pathology Tooth Germ / metabolism
IF 3.153
引用数 65
WOS 分野 TRANSPLANTATION MEDICINE, RESEARCH & EXPERIMENTAL HEMATOLOGY CELL & TISSUE ENGINEERING
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)