論文 - 詳細
| RRC ID | 18649 |
|---|---|
| 著者 | Takegawa Y, Araki K, Fujitani N, Furukawa J, Sugiyama H, Sakai H, Shinohara Y. |
| タイトル | Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography. |
| ジャーナル | Anal Chem |
| Abstract |
Glycosaminoglycans (GAGs) play important roles in cell adhesion and growth, maintenance of extracellular matrix (ECM) integrity, and signal transduction. To fully understand the biological functions of GAGs, there is a growing need for sensitive, rapid, and quantitative analysis of GAGs. The present work describes a novel analytical technique that enables high throughput cellular/tissue glycosaminoglycomics for all three families of uronic acid-containing GAGs, hyaluronan (HA), chondroitin sulfate (CS)/dermatan sulfate (DS), and heparan sulfate (HS). A one-pot purification and labeling procedure for GAG Δ-disaccharides was established by chemo-selective ligation of disaccharides onto high density hydrazide beads (glycoblotting) and subsequent labeling by fluorescence. The 17 most common disaccharides (eight comprising HS, eight CS/DS, and one comprising HA) could be separated with a single chromatography for the first time by employing a zwitter-ionic type of hydrophilic-interaction chromatography column. These novel analytical techniques were able to precisely characterize the glycosaminoglycome in various cell types including embryonal carcinoma cells and ocular epithelial tissues (cornea, conjunctiva, and limbus). |
| 巻・号 | 83(24) |
| ページ | 9443-9 |
| 公開日 | 2011-12-15 |
| DOI | 10.1021/ac2021079 |
| PMID | 22044073 |
| MeSH | Animals Cell Line Chondroitin Sulfates / analysis* Chromatography, High Pressure Liquid* Conjunctiva / metabolism Cricetinae Dermatan Sulfate / analysis* Disaccharides / analysis Heparitin Sulfate / analysis* Humans Hyaluronic Acid / analysis* Hydrophobic and Hydrophilic Interactions Ions / chemistry Mice Rabbits |
| IF | 6.785 |
| 引用数 | 33 |
| WOS 分野 | CHEMISTRY, ANALYTICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |