| RRC ID |
1867
|
| Author |
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A.
|
| Title |
The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis.
|
| Journal |
Plant Cell
|
| Abstract |
The outermost epidermal cell wall is specialized to withstand pathogens and natural stresses, and lipid-based cuticular polymers are the major barrier against incursions. The Arabidopsis thaliana mutant bodyguard (bdg), which exhibits defects characteristic of the loss of cuticle structure not attributable to a lack of typical cutin monomers, unexpectedly accumulates significantly more cell wall-bound lipids and epicuticular waxes than wild-type plants. Pleiotropic effects of the bdg mutation on growth, viability, and cell differentiation are also observed. BDG encodes a member of the alpha/beta-hydrolase fold protein superfamily and is expressed exclusively in epidermal cells. Using Strep-tag epitope-tagged BDG for mutant complementation and immunolocalization, we show that BDG is a polarly localized protein that accumulates in the outermost cell wall in the epidermis. With regard to the appearance and structure of the cuticle, the phenotype conferred by bdg is reminiscent of that of transgenic Arabidopsis plants that express an extracellular fungal cutinase, suggesting that bdg may be incapable of completing the polymerization of carboxylic esters in the cuticular layer of the cell wall or the cuticle proper. We propose that BDG codes for an extracellular synthase responsible for the formation of cuticle. The alternative hypothesis proposes that BDG controls the proliferation/differentiation status of the epidermis via an unknown mechanism.
|
| Volume |
18(2)
|
| Pages |
321-39
|
| Published |
2006-2-1
|
| DOI |
10.1105/tpc.105.036079
|
| PII |
tpc.105.036079
|
| PMID |
16415209
|
| PMC |
PMC1356542
|
| MeSH |
Amino Acid Sequence
Arabidopsis / anatomy & histology
Arabidopsis / growth & development*
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Cloning, Molecular
DNA Transposable Elements / genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genetic Complementation Test
Hydrolases / chemistry
Lipids / analysis
Molecular Sequence Data
Morphogenesis*
Organ Specificity
Phenotype
Plant Epidermis / growth & development*
Plant Epidermis / metabolism*
Plant Epidermis / ultrastructure
Plant Leaves / anatomy & histology
Plant Leaves / ultrastructure
Plants, Genetically Modified / metabolism
Protein Carbonylation
Protein Folding
Protein Transport
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Alignment
Waxes / metabolism
|
| IF |
9.618
|
| Times Cited |
179
|
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda12166
pda10970 |
