RRC ID 18702
著者 Uto-Kondo H, Ayaori M, Ogura M, Nakaya K, Ito M, Suzuki A, Takiguchi S, Yakushiji E, Terao Y, Ozasa H, Hisada T, Sasaki M, Ohsuzu F, Ikewaki K.
タイトル Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages.
ジャーナル Circ Res
Abstract RATIONALE:Association of habitual coffee consumption with coronary heart disease morbidity and mortality has not been established. We hypothesized that coffee may enhance reverse cholesterol transport (RCT) as the antiatherogenic properties of high-density lipoprotein (HDL).
OBJECTIVE:This study was to investigate whether the phenolic acids of coffee and coffee regulates RCT from macrophages in vitro, ex vivo and in vivo.
METHODS AND RESULTS:Caffeic acid and ferulic acid, the major phenolic acids of coffee, enhanced cholesterol efflux from THP-1 macrophages mediated by HDL, but not apoA-I. Furthermore, these phenolic acids increased both the mRNA and protein levels of ATP-binding cassette transporter (ABC)G1 and scavenger receptor class B type I (SR-BI), but not ABCA1. Eight healthy volunteers were recruited for the ex vivo study, and blood samples were taken before and 30 minutes after consumption of coffee or water in a crossover study. The mRNA as well as protein levels of ABCG1, SR-BI, and cholesterol efflux by HDL were increased in the macrophages differentiated under autologous sera obtained after coffee consumption compared to baseline sera. Finally, effects of coffee and phenolic acid on in vivo RCT were assessed by intraperitoneally injecting [(3)H]cholesterol-labeled acetyl low-density lipoprotein-loaded RAW264.7 cells into mice, then monitoring appearance of (3)H tracer in plasma, liver, and feces. Supporting in vitro and ex vivo data, ferulic acid was found to significantly increase the levels of (3)H tracer in feces.
CONCLUSIONS:Coffee intake might have an antiatherogenic property by increasing ABCG1 and SR-BI expression and enhancing HDL-mediated cholesterol efflux from the macrophages via its plasma phenolic acids.
巻・号 106(4)
ページ 779-87
公開日 2010-3-5
DOI 10.1161/CIRCRESAHA.109.206615
PII CIRCRESAHA.109.206615
PMID 20075335
MeSH ATP Binding Cassette Transporter 1 ATP Binding Cassette Transporter, Subfamily G, Member 1 ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism Adult Animals Apolipoprotein A-I / metabolism Beverages* Bile / metabolism Biological Transport Caffeic Acids / blood Caffeic Acids / pharmacology* Cell Line Cholesterol / blood Cholesterol / metabolism* Coffee* Coronary Disease / metabolism Coronary Disease / prevention & control Coumaric Acids / blood Coumaric Acids / pharmacology* Cross-Over Studies Dose-Response Relationship, Drug Feces / chemistry Female Genes, Reporter Humans Lipoproteins, HDL / blood Lipoproteins, HDL / metabolism* Lipoproteins, LDL / metabolism Liver / metabolism Macrophages / drug effects* Macrophages / metabolism Male Mice Mice, Inbred C57BL Middle Aged RNA, Messenger / metabolism Scavenger Receptors, Class B / genetics Scavenger Receptors, Class B / metabolism Time Factors Transfection Up-Regulation
IF 14.467
引用数 58
WOS 分野 CARDIAC & CARDIOVASCULAR SYSTEMS PERIPHERAL VASCULAR DISEASE HEMATOLOGY
リソース情報
ヒト・動物細胞 THP-1(RCB1189)