RRC ID 18717
Author Suzuki T, Chang MO, Kitajima M, Takaku H.
Title Baculovirus activates murine dendritic cells and induces non-specific NK cell and T cell immune responses.
Journal Cell Immunol
Abstract We previously reported that the baculovirus induced a strong host immune response against infections and malignancies. Among the immune cells, the dendritic cells were most strongly infected and activated by the baculovirus, although the exact mechanism remained unclear. Here, we evaluated the non-specific immune responses of bone marrow-derived dendritic cells (BMDCs) after infection by a wild-type baculovirus. MHC class I and II molecules and co-stimulation molecules (CD40, CD80, and CD86) on BMDCs were up-regulated by baculovirus infection. At the same time, the BMDCs produced pre-inflammatory cytokines (IL-6, IL12p70, and TNF-alpha) and IFN-alpha. NK cells showed IFN-gamma production, CD69 up-regulation, and enhanced cytotoxicity when they were co-cultured with baculovirus-infected BMDCs. T cells showed IFN-gamma production, CD69 up-regulation, and cell proliferation. Ex vivo analysis performed in vitro produced similar results. These findings suggested that baculovirus-infected dendritic cells induce non-specific immune responses and can be used as an immunotherapeutic agent against viral infections and malignancies, together with present therapeutic drug regimens.
Volume 262(1)
Pages 35-43
Published 2010-1-1
DOI 10.1016/j.cellimm.2009.12.005
PII S0008-8749(09)00225-1
PMID 20060108
MeSH Animals Antigens, CD / immunology Baculoviridae / genetics Baculoviridae / immunology* Bone Marrow / immunology Cell Differentiation Cell Proliferation Cells, Cultured Coculture Techniques Cytokines / biosynthesis Cytokines / immunology DNA, Viral / genetics Dendritic Cells / cytology Dendritic Cells / immunology* Female Gene Expression Regulation, Viral Killer Cells, Natural / immunology* Lymphocyte Activation Mice Mice, Inbred C57BL T-Lymphocytes / immunology* Up-Regulation
IF 4.078
Times Cited 35
Human and Animal Cells RAW 264(RCB0535)