論文 - 詳細
| RRC ID | 18735 |
|---|---|
| 著者 | Sugawara Y, Matsumura T, Takegahara Y, Jin Y, Tsukasaki Y, Takeichi M, Fujinaga Y. |
| タイトル | Botulinum hemagglutinin disrupts the intercellular epithelial barrier by directly binding E-cadherin. |
| ジャーナル | J Cell Biol |
| Abstract |
Botulinum neurotoxin is produced by Clostridium botulinum and forms large protein complexes through associations with nontoxic components. We recently found that hemagglutinin (HA), one of the nontoxic components, disrupts the intercellular epithelial barrier; however, the mechanism underlying this phenomenon is not known. In this study, we identified epithelial cadherin (E-cadherin) as a target molecule for HA. HA directly binds E-cadherin and disrupts E-cadherin-mediated cell to cell adhesion. Although HA binds human, bovine, and mouse E-cadherin, it does not bind rat or chicken E-cadherin homologues. HA does not interact with other members of the classical cadherin family such as neural and vascular endothelial cadherin. Expression of rat E-cadherin but not mouse rescues Madin-Darby canine kidney cells from HA-induced tight junction (TJ) disruptions. These data demonstrate that botulinum HA directly binds E-cadherin and disrupts E-cadherin-mediated cell to cell adhesion in a species-specific manner and that the HA-E-cadherin interaction is essential for the disruption of TJ function. |
| 巻・号 | 189(4) |
| ページ | 691-700 |
| 公開日 | 2010-5-17 |
| DOI | 10.1083/jcb.200910119 |
| PII | jcb.200910119 |
| PMID | 20457762 |
| PMC | PMC2872904 |
| MeSH | Animals Botulinum Toxins / metabolism* Botulinum Toxins / pharmacology Cadherins / chemistry Cadherins / metabolism Cattle Cell Adhesion / drug effects Cell Line Dogs Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / metabolism Hemagglutinins / metabolism* Hemagglutinins / pharmacology Humans Mice Rats |
| IF | 8.811 |
| 引用数 | 67 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 62 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDBK(RCB0180) NBT-T1(RCB1369) |