RRC ID 18745
著者 Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Fujita S, Miyake M, Miyake J.
タイトル Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.
ジャーナル Methods Mol Biol
Abstract Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
巻・号 629
ページ 193-203
公開日 2010-1-1
DOI 10.1007/978-1-60761-657-3_13
PMID 20387151
MeSH Biological Assay Cell Movement / drug effects Cell Movement / genetics* Collagen Type I / pharmacology Fibronectins / metabolism HeLa Cells High-Throughput Screening Assays / methods* Humans Oligonucleotide Array Sequence Analysis / methods* Phenotype Rhodamines / metabolism Statistics as Topic Transfection / methods*
引用数 3
リソース情報
ヒト・動物細胞 NBT-L2b(RCB1373)