| RRC ID |
18894
|
| 著者 |
Xiao L, Aoshima H, Saitoh Y, Miwa N.
|
| タイトル |
The effect of squalane-dissolved fullerene-C60 on adipogenesis-accompanied oxidative stress and macrophage activation in a preadipocyte-monocyte co-culture system.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Effects of squalane-dissolved fullerene-C60 (Sql-fullerene) on macrophage activation and adipose conversion with oxidative stress were studied using an inflammatory adipose-tissue equivalent (ATE) and OP9 mouse stromal preadipocyte-U937 lymphoma cell co-culture systems. Differentiation of OP9 cells was initiated by insulin-rich serum replacement (SR) as an adipogenic stimulant, and then followed by accumulation of intracellular lipid droplets and reactive oxygen species (ROS), both of which were significantly inhibited by Sql-fullerene. In the OP9-U937 cell co-culture system, U937 cells rapidly differentiated to macrophage-like cells during SR-induced adipogenesis in OP9 cells. The ROS accumulation was in the co-culture more marked than in OP9 cells alone, suggesting that the interaction between adipocytes and monocytes/macrophages promotes inflammatory responses. Sql-fullerene significantly inhibited macrophage activation and low-grade adipogenesis in the OP9-U937 co-culture system. We developed a three-dimensional inflammatory adipose-tissue model "ATE" consisting of, characteristically, U937 cells in the culture-wells, and, in addition, mounted a culture insert containing OP9 cells-populated collagen gel. ATE is enabled with suitable stimulation to represent the pathology of inflammatory disorders, such as macrophage infiltration in adipose tissue. Five-day culturing of ATE in SR medium occurred U937 macrophage migration and intracellular oil-droplet accumulation that were significantly inhibited by Sql-fullerene. Our results suggest that Sql-fullerene might be explored as a potential medicine for the treatment of metabolic syndrome or other obesity-related disorders.
|
| 巻・号 |
31(23)
|
| ページ |
5976-85
|
| 公開日 |
2010-8-1
|
| DOI |
10.1016/j.biomaterials.2010.04.032
|
| PII |
S0142-9612(10)00548-X
|
| PMID |
20488530
|
| MeSH |
Adipocytes / metabolism
Adipogenesis*
Animals
Cell Differentiation
Cell Line
Cell Line, Tumor
Coculture Techniques
Fullerenes / chemistry*
Humans
Macrophage Activation*
Mice
Microscopy, Electron, Scanning
Oxidative Stress*
Reactive Oxygen Species / metabolism
Squalene / chemistry*
|
| IF |
10.317
|
| 引用数 |
24
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
OP9(RCB1124) |
