RRC ID 1891
Author Desaki Y, Miya A, Venkatesh B, Tsuyumu S, Yamane H, Kaku H, Minami E, Shibuya N.
Title Bacterial lipopolysaccharides induce defense responses associated with programmed cell death in rice cells.
Journal Plant Cell Physiol.
Abstract PAMP (pathogen-associated molecular pattern) recognition plays an important role during the innate immune response in both plants and animals. Lipopolysaccharides (LPS) derived from Gram-negative bacteria are representative of typical PAMP molecules and have been reported to induce defense-related responses, including the suppression of the hypersensitive response, the expression of defense genes and systemic resistance in plants. However, the details regarding the precise molecular mechanisms underlying these cellular responses, such as the molecular machinery involved in the perception and transduction of LPS molecules, remain largely unknown. Furthermore, the biological activities of LPS on plants have so far been reported only in dicots and no information is thus available regarding their functions in monocots. In our current study, we report that LPS preparations for various becteria, including plant pathogens and non-pathogens, can induce defense responses in rice cells, including reactive oxygen generation and defense gene expression. In addition, global analysis of gene expression induced by two PAMPs, LPS and chitin oligosaccharide, also reveals a close correlation between the gene responses induced by these factors. This indicates that there is a convergence of signaling cascades downstream of their corresponding receptors. Furthermore, we show that the defense responses induced by LPS in the rice cells are associated with programmed cell death (PCD), which is a finding that has not been previously reported for the functional role of these molecules in plant cells. Interestingly, PCD induction by the LPS was not detected in cultured Arabidopsis thaliana cells.
Volume 47(11)
Pages 1530-40
Published 2006-11
DOI 10.1093/pcp/pcl019
PII pcl019
PMID 17018557
MeSH Adsorption Apoptosis / drug effects* Arabidopsis / cytology Arabidopsis / drug effects Chitin / pharmacology Cycloheximide / pharmacology DNA Fragmentation / drug effects Down-Regulation / drug effects Gene Expression Regulation, Plant / drug effects Genes, Plant In Situ Nick-End Labeling Lipopolysaccharides / pharmacology* Oryza / cytology* Oryza / drug effects* Oryza / genetics Oryza / immunology Polymyxin B / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Reactive Oxygen Species / metabolism Sepharose / metabolism Up-Regulation / drug effects
IF 4.059
Times Cited 65
WOS Category PLANT SCIENCES CELL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes rpc00008