RRC ID |
18969
|
著者 |
Arano T, Sato T, Matsuzaka K, Ikada Y, Yoshinari M.
|
タイトル |
Osteoblastic cell proliferation with uniform distribution in a large scaffold using radial-flow bioreactor.
|
ジャーナル |
Tissue Eng Part C Methods
|
Abstract |
Bioreactors employing different types of in vitro physiological cell stimulation have been developed to obtain three-dimensional cultivation for tissue engineering. The aim of this study was to determine whether osteoblastic cells proliferated uniformly over a large scaffold with a diameter of 18 mm and height of 10 mm under dynamic cultivation with the radial-flow bioreactor (RFB), and thereby ascertain the potential of this system in the regeneration of jaw bone. Mouse osteoblastic cells (MC3T3-E1) were seeded onto type 1 collagen sheets. Cells were then incubated outside the reactor for 6 h to produce precultured sheets. The six precultured sheets were then placed in the RFB to fabricate the scaffolds. Cells were dynamically cultured for 1 week at 37°C, pH 7.4, dissolved oxygen 6.86 ppm, and with the culture medium circulating at 3 mL/min. As a control, static cultivation cultured in the same manner without the circulating culture medium and single cultivation in a culture dish were performed. The resulting cell proliferation and cell distribution were analyzed. After 1 week of dynamic cultivation, cells showed a fivefold and fourfold increase with uniform distribution throughout the three-dimensional scaffolds than those under the static and single cultivation as a control, respectively. These results indicate that the RFB is a promising system for three-dimensional cultivation of osteoblastic cells for treating large bone defects by tissue engineering.
|
巻・号 |
16(6)
|
ページ |
1387-98
|
公開日 |
2010-12-1
|
DOI |
10.1089/ten.TEC.2009.0377
|
PMID |
20367244
|
MeSH |
Animals
Bioreactors*
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods
Cell Proliferation*
Cells, Cultured
DNA / analysis
DNA / metabolism
Mice
Models, Biological
Osteoblasts / cytology*
Osteoblasts / metabolism
Osteoblasts / physiology*
Staining and Labeling
Tissue Engineering / instrumentation
Tissue Engineering / methods
Tissue Scaffolds* / chemistry
|
IF |
3.508
|
引用数 |
8
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126) |