論文 - 詳細
| RRC ID | 18969 |
|---|---|
| 著者 | Arano T, Sato T, Matsuzaka K, Ikada Y, Yoshinari M. |
| タイトル | Osteoblastic cell proliferation with uniform distribution in a large scaffold using radial-flow bioreactor. |
| ジャーナル | Tissue Eng Part C Methods |
| Abstract |
Bioreactors employing different types of in vitro physiological cell stimulation have been developed to obtain three-dimensional cultivation for tissue engineering. The aim of this study was to determine whether osteoblastic cells proliferated uniformly over a large scaffold with a diameter of 18 mm and height of 10 mm under dynamic cultivation with the radial-flow bioreactor (RFB), and thereby ascertain the potential of this system in the regeneration of jaw bone. Mouse osteoblastic cells (MC3T3-E1) were seeded onto type 1 collagen sheets. Cells were then incubated outside the reactor for 6 h to produce precultured sheets. The six precultured sheets were then placed in the RFB to fabricate the scaffolds. Cells were dynamically cultured for 1 week at 37°C, pH 7.4, dissolved oxygen 6.86 ppm, and with the culture medium circulating at 3 mL/min. As a control, static cultivation cultured in the same manner without the circulating culture medium and single cultivation in a culture dish were performed. The resulting cell proliferation and cell distribution were analyzed. After 1 week of dynamic cultivation, cells showed a fivefold and fourfold increase with uniform distribution throughout the three-dimensional scaffolds than those under the static and single cultivation as a control, respectively. These results indicate that the RFB is a promising system for three-dimensional cultivation of osteoblastic cells for treating large bone defects by tissue engineering. |
| 巻・号 | 16(6) |
| ページ | 1387-98 |
| 公開日 | 2010-12-1 |
| DOI | 10.1089/ten.TEC.2009.0377 |
| PMID | 20367244 |
| MeSH | Animals Bioreactors* Cell Culture Techniques / instrumentation Cell Culture Techniques / methods Cell Proliferation* Cells, Cultured DNA / analysis DNA / metabolism Mice Models, Biological Osteoblasts / cytology* Osteoblasts / metabolism Osteoblasts / physiology* Staining and Labeling Tissue Engineering / instrumentation Tissue Engineering / methods Tissue Scaffolds* / chemistry |
| IF | 3.508 |
| 引用数 | 8 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |