RRC ID 1898
Author Cao F, Hata R, Zhu P, Ma YJ, Tanaka J, Hanakawa Y, Hashimoto K, Niinobe M, Yoshikawa K, Sakanaka M.
Title Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells.
Journal J Neurochem
Abstract To investigate the effects of suppressors of cytokine signaling 3 (SOCS3) on neural stem cell fate, stem cells were infected with an adenoviral vector expressing SOCS3. Three days later, western blot analysis and immunocytochemical analysis revealed that the protein level of MAP2 and the number of MAP2-positive cells were significantly increased in SOCS3-transfected cells, whereas the protein level of GFAP and the number of GFAP-positive cells were significantly decreased. Furthermore, promoter assay revealed a significant reduction in the transcriptional level of signal transducer and activator of transcription 3 (Stat3) in the transfected cells. In addition, the mRNA levels of Notch family member (notch1) and inhibitory basic helix-loop-helix (bHLH) factors (hes5 and id3) were significantly up-regulated 1 day after overexpression of SOCS3. Three days after transfection, the mRNA level of hes5 was significantly decreased, whereas that of notch1 was still up-regulated. Moreover, all of SOCS3-positive cells expressed Nestin protein but did not express MAP2 or GFAP proteins. These data indicate that overexpression of SOCS3 induced neurogenesis and inhibited astrogliogenesis in neural stem cells. Our data also show that SOCS3 promoted maintenance of neural stem cells.
Volume 98(2)
Pages 459-70
Published 2006-7-1
DOI 10.1111/j.1471-4159.2006.03890.x
PMID 16805839
MeSH Adenoviridae / metabolism Astrocytes / drug effects* Cell Differentiation / drug effects Genetic Vectors Glial Fibrillary Acidic Protein / biosynthesis Helix-Loop-Helix Motifs / genetics Humans Immunoblotting Immunohistochemistry Leukocyte Migration-Inhibitory Factors / pharmacology Luciferases / metabolism Neurons / drug effects* Promoter Regions, Genetic / genetics Receptor, Notch1 / biosynthesis Receptor, Notch1 / genetics Reverse Transcriptase Polymerase Chain Reaction STAT3 Transcription Factor / physiology Signal Transduction / physiology Stem Cells / drug effects* Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins / biosynthesis* Suppressor of Cytokine Signaling Proteins / genetics* Up-Regulation
IF 4.066
Times Cited 29
DNA material pAxCAwt (RDB1678)